This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Venkatesh, T V Articles by Radding, C M Search for Related Content PubMed PubMed Citation Articles by Venkatesh, T V Articles by Radding, C M

research-article

Ribosomal protein S1 and NusA protein complexed to recombination protein beta of phage lambda.

Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510.

ABSTRACT

The red genes of bacteriophage lambda specify two proteins, exonuclease and beta protein, which are essential for general recombination of lambda in recA cells. Earlier studies suggested that these proteins form an equimolar complex (C. M. Radding, J. Rosenweig, F. Richards, and E. Cassuto, J. Biol. Chem. 246:2510-2512, 1971). A more recent study indicated that beta protein forms a strong complex with an unknown polypeptide of 70 kDa (K. Muniyappa and C. M. Radding, J. Biol. Chem. 261:7472-7478, 1986). In the present study, in addition to the complex of beta and the 70-kDa protein, a new association of beta protein with a 65-kDa protein was observed. N-terminal sequencing identified these proteins as host-encoded ribosomal protein S1 and transcription terminator protein NusA.

This article has been cited by other articles:

• Skorski, P., Proux, F., Cheraiti, C., Dreyfus, M., Denmat, S. H.-L. (2007). The Deleterious Effect of an Insertion Sequence Removing the Last Twenty Percent of the Essential Escherichia coli rpsA Gene Is Due to mRNA Destabilization, Not Protein Truncation. J. Bacteriol. 189: 6205-6212 [Abstract] [Full Text]  
• Sukhodolets, M. V., Garges, S., Adhya, S. (2006). Ribosomal protein S1 promotes transcriptional cycling. RNA 12: 1505-1513 [Abstract] [Full Text]  
• Boni, I. V., Artamonova, V. S., Dreyfus, M. (2000). The Last RNA-Binding Repeat of the Escherichia coli Ribosomal Protein S1 Is Specifically Involved in Autogenous Control. J. Bacteriol. 182: 5872-5879 [Abstract] [Full Text]  
• Kuzminov, A. (1999). Recombinational Repair of DNA Damage in Escherichia coli and Bacteriophage lambda. Microbiol. Mol. Biol. Rev. 63: 751-813 [Abstract] [Full Text]  
• Mogridge, J., Greenblatt, J. (1998). Specific Binding of Escherichia coli Ribosomal Protein S1 to boxA Transcriptional Antiterminator RNA. J. Bacteriol. 180: 2248-2252 [Abstract] [Full Text]