This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Mierau, I Articles by Venema, G Search for Related Content PubMed PubMed Citation Articles by Mierau, I Articles by Venema, G

research-article

Cloning and sequencing of the gene for a lactococcal endopeptidase, an enzyme with sequence similarity to mammalian enkephalinase.

Department of Genetics, University of Groningen, Haren, The Netherlands.

ABSTRACT

The gene specifying an endopeptidase of Lactococcus lactis, named pepO, was cloned from a genomic library of L. lactis subsp. cremoris P8-2-47 in lambda EMBL3 and was subsequently sequenced. pepO is probably the last gene of an operon encoding the binding-protein-dependent oligopeptide transport system of L. lactis. The inferred amino acid sequence of PepO showed that the lactococcal endopeptidase has a marked similarity to the mammalian neutral endopeptidase EC 3.4.24.11 (enkephalinase), whereas no obvious sequence similarity with any bacterial enzyme was found. By means of gene disruption, a pepO-negative mutant was constructed. Growth and acid production of the mutant strain in milk were not affected, indicating that the endopeptidase is not essential for growth of L. lactis in milk.

This article has been cited by other articles:

• Janer, C., Arigoni, F., Lee, B. H., Pelaez, C., Requena, T. (2005). Enzymatic Ability of Bifidobacterium animalis subsp. lactis To Hydrolyze Milk Proteins: Identification and Characterization of Endopeptidase O. Appl. Environ. Microbiol. 71: 8460-8465 [Abstract] [Full Text]  
• Vido, K., le Bars, D., Mistou, M.-Y., Anglade, P., Gruss, A., Gaudu, P. (2004). Proteome Analyses of Heme-Dependent Respiration in Lactococcus lactis: Involvement of the Proteolytic System. J. Bacteriol. 186: 1648-1657 [Abstract] [Full Text]  
• Chen, Y.-S., Christensen, J. E., Broadbent, J. R., Steele, J. L. (2003). Identification and Characterization of Lactobacillus helveticus PepO2, an Endopeptidase with Post-Proline Specificity. Appl. Environ. Microbiol. 69: 1276-1282 [Abstract] [Full Text]  
• Tuler, T. R., Callanan, M. J., Klaenhammer, T. R. (2002). Overexpression of Peptidases in Lactococcus and Evaluation of Their Release from Leaky Cells. J DAIRY SCI 85: 2438-2450 [Abstract] [Full Text]  
• Berge, M., Langen, H., Claverys, J.-P., Martin, B. (2002). Identification of a Protein That Inactivates the Competence-Stimulating Peptide of Streptococcus pneumoniae. J. Bacteriol. 184: 610-613 [Abstract] [Full Text]  
• Christensson, C., Bratt, H., Collins, L. J., Coolbear, T., Holland, R., Lubbers, M. W., O'Toole, P. W., Reid, J. R. (2002). Cloning and Expression of an Oligopeptidase, PepO, with Novel Specificity from Lactobacillus rhamnosus HN001 (DR20). Appl. Environ. Microbiol. 68: 254-262 [Abstract] [Full Text]  
• Guédon, E., Renault, P., Ehrlich, S. D., Delorme, C. (2001). Transcriptional Pattern of Genes Coding for the Proteolytic System of Lactococcus lactis and Evidence for Coordinated Regulation of Key Enzymes by Peptide Supply. J. Bacteriol. 183: 3614-3622 [Abstract] [Full Text]  
• Oetjen, J., Fives-Taylor, P., Froeliger, E. (2001). Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme. Infect. Immun. 69: 58-64 [Abstract] [Full Text]  
• Dufour, A., Rincé, A., Uguen, P., Le Pennec, J.-P. (2000). IS1675, a Novel Lactococcal Insertion Element, Forms a Transposon-Like Structure Including the Lacticin 481 Lantibiotic Operon. J. Bacteriol. 182: 5600-5605 [Abstract] [Full Text]  
• Froeliger, E. H., Oetjen, J., Bond, J. P., Fives-Taylor, P. (1999). Streptococcus parasanguis pepO Encodes an Endopeptidase with Structure and Activity Similar to Those of Enzymes That Modulate Peptide Receptor Signaling in Eukaryotic Cells. Infect. Immun. 67: 5206-5214 [Abstract] [Full Text]  
• Tiraboschi, G., Jullian, N., Thery, V., Antonczak, S., Fournie-Zaluski, M.-C., Roques, B. P. (1999). A three-dimensional construction of the active site (region 507–749) of human neutral endopeptidase (EC.3.4.24.11). Protein Eng Des Sel 12: 141-149 [Abstract] [Full Text]  
• Chen, Y.-S., Steele, J. L. (1998). Genetic Characterization and Physiological Role of Endopeptidase O from Lactobacillus helveticus CNRZ32. Appl. Environ. Microbiol. 64: 3411-3415 [Abstract] [Full Text]  
• Valdenaire, O., Rohrbacher, E., Mattei, M.-G.èv. (1995). Organization of the Gene Encoding the Human Endothelin-converting Enzyme (ECE-1). J. Biol. Chem. 270: 29794-29798 [Abstract] [Full Text]