This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McCann, M P Articles by Matin, A Search for Related Content PubMed PubMed Citation Articles by McCann, M P Articles by Matin, A

research-article

The putative sigma factor KatF is regulated posttranscriptionally during carbon starvation.

Department of Microbiology and Immunology, Stanford University, California 94305.

ABSTRACT

Transcriptional and translational 'lacZ reporter fusions were constructed to the katF gene, which encodes a putative sigma factor centrally involved in starvation-mediated general resistance in Escherichia coli. Transcription of katF was found to increase ca. twofold after carbon starvation in minimal medium. The protein fusion containing the longest fragment of katF induced ca. eightfold under the same conditions, whereas fusions to shorter segments showed only a twofold increase in expression. The protein fusion was expressed at higher levels in a strain containing a katF::Tn10 mutation, indicating katF autoregulation. The posttranscriptional regulation of katF by starvation did not require a component of the spent minimal medium. katF was also posttranscriptionally regulated during entry into late log phase in complex medium. This induction was coincident with an increase in katE transcription, suggesting that the cellular concentration of KatF directly followed the induction of the katF protein fusion.

This article has been cited by other articles:

• Bougdour, A., Wickner, S., Gottesman, S. (2006). Modulating RssB activity: IraP, a novel regulator of {sigma}S stability in Escherichia coli.. Genes Dev. 20: 884-897 [Abstract] [Full Text]  
• Peterson, C. N., Mandel, M. J., Silhavy, T. J. (2005). Escherichia coli Starvation Diets: Essential Nutrients Weigh in Distinctly. J. Bacteriol. 187: 7549-7553 [Full Text]  
• Hirsch, M., Elliott, T. (2005). Stationary-Phase Regulation of RpoS Translation in Escherichia coli. J. Bacteriol. 187: 7204-7213 [Abstract] [Full Text]  
• Nishino, K., Honda, T., Yamaguchi, A. (2005). Genome-Wide Analyses of Escherichia coli Gene Expression Responsive to the BaeSR Two-Component Regulatory System. J. Bacteriol. 187: 1763-1772 [Abstract] [Full Text]  
• Mandel, M. J., Silhavy, T. J. (2005). Starvation for Different Nutrients in Escherichia coli Results in Differential Modulation of RpoS Levels and Stability. J. Bacteriol. 187: 434-442 [Abstract] [Full Text]  
• Lynch, S. V., Brodie, E. L., Matin, A. (2004). Role and Regulation of {sigma}s in General Resistance Conferred by Low-Shear Simulated Microgravity in Escherichia coli. J. Bacteriol. 186: 8207-8212 [Abstract] [Full Text]  
• Park, K.-J., Kang, M.-J., Kim, S. H., Lee, H.-J., Lim, J.-K., Choi, S. H., Park, S.-J., Lee, K.-H. (2004). Isolation and Characterization of rpoS from a Pathogenic Bacterium, Vibrio vulnificus: Role of {sigma}S in Survival of Exponential-Phase Cells under Oxidative Stress. J. Bacteriol. 186: 3304-3312 [Abstract] [Full Text]  
• Ihssen, J., Egli, T. (2004). Specific growth rate and not cell density controls the general stress response in Escherichia coli. Microbiology 150: 1637-1648 [Abstract] [Full Text]  
• Nishino, K., Yamaguchi, A. (2004). Role of Histone-Like Protein H-NS in Multidrug Resistance of Escherichia coli. J. Bacteriol. 186: 1423-1429 [Abstract] [Full Text]  
• Mallik, P., Pratt, T. S., Beach, M. B., Bradley, M. D., Undamatla, J., Osuna, R. (2004). Growth Phase-Dependent Regulation and Stringent Control of fis Are Conserved Processes in Enteric Bacteria and Involve a Single Promoter (fis P) in Escherichia coli. J. Bacteriol. 186: 122-135 [Abstract] [Full Text]  
• Arai, A., Kawachi, E., Hata, M., Ogura, M., Tanaka, T. (2003). Inhibition of Bacillus subtilis aprE Expression by Lincomycin at the Posttranscriptional Level through Inhibition of ppGpp Synthesis. J Biochem 134: 691-697 [Abstract] [Full Text]  
• Nishino, K., Inazumi, Y., Yamaguchi, A. (2003). Global Analysis of Genes Regulated by EvgA of the Two-Component Regulatory System in Escherichia coli. J. Bacteriol. 185: 2667-2672 [Abstract] [Full Text]  
• Hirsch, M., Elliott, T. (2002). Role of ppGpp in rpoS Stationary-Phase Regulation in Escherichia coli. J. Bacteriol. 184: 5077-5087 [Abstract] [Full Text]  
• Hengge-Aronis, R. (2002). Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase. Microbiol. Mol. Biol. Rev. 66: 373-395 [Abstract] [Full Text]  
• Seshadri, R., Samuel, J. E. (2001). Characterization of a Stress-Induced Alternate Sigma Factor, RpoS, of Coxiella burnetii and Its Expression during the Development Cycle. Infect. Immun. 69: 4874-4883 [Abstract] [Full Text]  
• Sledjeski, D. D., Whitman, C., Zhang, A. (2001). Hfq Is Necessary for Regulation by the Untranslated RNA DsrA. J. Bacteriol. 183: 1997-2005 [Abstract] [Full Text]  
• Li, C., Tao, Y. P., Simon, L. D. (2000). Expression of Different-Size Transcripts from the clpP-clpX Operon of Escherichia coli during Carbon Deprivation. J. Bacteriol. 182: 6630-6637 [Abstract] [Full Text]  
• Yu, S.-M. (1999). Cellular and Genetic Responses of Plants to Sugar Starvation. Plant Physiol. 121: 687-693 [Full Text]  
• Mason, C. A., Dünner, J., Indra, P., Colangelo, T. (1999). Heat-Induced Expression and Chemically Induced Expression of the Escherichia coli Stress Protein HtpG Are Affected by the Growth Environment. Appl. Environ. Microbiol. 65: 3433-3440 [Abstract] [Full Text]  
• Hu, Y., Coates, A. R. M. (1999). Transcription of the Stationary-Phase-Associated hspX Gene of Mycobacterium tuberculosis Is Inversely Related to Synthesis of the 16-Kilodalton Protein. J. Bacteriol. 181: 1380-1387 [Abstract] [Full Text]  
• Gibson, K. E., Silhavy, T. J. (1999). The LysR Homolog LrhA Promotes RpoS Degradation by Modulating Activity of the Response Regulator SprE. J. Bacteriol. 181: 563-571 [Abstract] [Full Text]  
• Schellhorn, H. E., Audia, J. P., Wei, L. I. C., Chang, L. (1998). Identification of Conserved, RpoS-Dependent Stationary-Phase Genes of Escherichia coli. J. Bacteriol. 180: 6283-6291 [Abstract] [Full Text]  
• Rockabrand, D., Livers, K., Austin, T., Kaiser, R., Jensen, D., Burgess, R., Blum, P. (1998). Roles of DnaK and RpoS in Starvation-Induced Thermotolerance of Escherichia coli. J. Bacteriol. 180: 846-854 [Abstract] [Full Text]  
• Muffler, A, Fischer, D, Hengge-Aronis, R (1996). The RNA-binding protein HF-I, known as a host factor for phage Qbeta RNA replication, is essential for rpoS translation in Escherichia coli.. Genes Dev. 10: 1143-1151 [Abstract]  
• Huisman, G., Kolter, R (1994). Sensing starvation: a homoserine lactone--dependent signaling pathway in Escherichia coli. Science 265: 537-539 [Abstract]  
• Lange, R, Hengge-Aronis, R (1994). The cellular concentration of the sigma S subunit of RNA polymerase in Escherichia coli is controlled at the levels of transcription, translation, and protein stability.. Genes Dev. 8: 1600-1612 [Abstract]