This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Frustaci, J M Articles by O'Brian, M R Search for Related Content PubMed PubMed Citation Articles by Frustaci, J M Articles by O'Brian, M R

research-article

The Escherichia coli visA gene encodes ferrochelatase, the final enzyme of the heme biosynthetic pathway.

Department of Biochemistry, State University of New York, Buffalo 14214.

ABSTRACT

An Escherichia coli mutant with a disrupted visA gene was defective in ferrochelatase activity but expressed wild-type levels of protoporphyrinogen oxidase activity. The visA coding region was placed under the transcriptional control of T7 RNA polymerase in an E. coli expression system, and the product was expressed as a 38-kDa protein. The overexpressed protein was purified to near homogeneity and was found to contain ferrochelatase activity. The data show that the visA gene encodes ferrochelatase, and we propose that it be renamed hemH to reflect that conclusion.

This article has been cited by other articles:

• Najahi-Missaoui, W., Dailey, H. A. (2005). Production and characterization of erythropoietic protoporphyric heterodimeric ferrochelatases. Blood 106: 1098-1104 [Abstract] [Full Text]  
• Feissner, R. E., Beckett, C. S., Loughman, J. A., Kranz, R. G. (2005). Mutations in Cytochrome Assembly and Periplasmic Redox Pathways in Bordetella pertussis. J. Bacteriol. 187: 3941-3949 [Abstract] [Full Text]  
• Tibor, A., Wansard, V., Bielartz, V., Delrue, R.-M., Danese, I., Michel, P., Walravens, K., Godfroid, J., Letesson, J.-J. (2002). Effect of omp10 or omp19 Deletion on Brucella abortus Outer Membrane Properties and Virulence in Mice. Infect. Immun. 70: 5540-5546 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Sellers, V. M., Wang, K.-F., Johnson, M. K., Dailey, H. A. (1998). Evidence That the Fourth Ligand to the [2Fe-2S] Cluster in Animal Ferrochelatase Is a Cysteine. CHARACTERIZATION OF THE ENZYME FROM DROSOPHILA MELANOGASTER. J. Biol. Chem. 273: 22311-22316 [Abstract] [Full Text]  
• Nishimura, K., Taketani, S., Inokuchi, H. (1995). Cloning of a Human cDNA for Protoporphyrinogen Oxidase by Complementation in Vivo of a hemG Mutant of Escherichia coli. J. Biol. Chem. 270: 8076-8080 [Abstract] [Full Text]