This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Prieto, M A Articles by Garcia, J L Search for Related Content PubMed PubMed Citation Articles by Prieto, M A Articles by Garcia, J L

research-article

Characterization of an Escherichia coli aromatic hydroxylase with a broad substrate range.

Unidad de Genética Bacteriana, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientificas, Velázquez, Madrid.

ABSTRACT

The hpaB gene encoding an aromatic hydroxylase of Escherichia coli ATCC 11105, a penicillin G acylase-producing strain, has been cloned and expressed in E. coli K-12. This gene was located near the pacA gene coding for penicillin G acylase. The hydroxylase has a molecular mass of 59,000 Da, uses NADH as a cosubstrate, and was tentatively classified as a 4-hydroxyphenylacetic acid hydroxylase, albeit it exhibited a rather broad substrate specificity acting on different monohydric and dihydric phenols. E. coli W, C, and B as well as Klebsiella pneumoniae M5a1 and Kluyvera citrophila ATCC 21285 (a penicillin G acylase-producing strain) but not E. coli K-12 contained sequences homologous to hpaB. Our results support the hypothesis that hpaB is a component of the 4-hydroxyphenylacetic acid degradative pathway of E. coli W.

This article has been cited by other articles:

• Galan, B., Manso, I., Kolb, A., Garcia, J. L., Prieto, M. A. (2008). The role of FIS protein in the physiological control of the expression of the Escherichia coli meta-hpa operon. Microbiology 154: 2151-2160 [Abstract] [Full Text]  
• Nijvipakul, S., Wongratana, J., Suadee, C., Entsch, B., Ballou, D. P., Chaiyen, P. (2008). LuxG Is a Functioning Flavin Reductase for Bacterial Luminescence. J. Bacteriol. 190: 1531-1538 [Abstract] [Full Text]  
• Huang, Y., Leming, C. L., Suyemoto, M., Altier, C. (2007). Genome-Wide Screen of Salmonella Genes Expressed during Infection in Pigs, Using In Vivo Expression Technology. Appl. Environ. Microbiol. 73: 7522-7530 [Abstract] [Full Text]  
• Arias-Barrau, E., Sandoval, A., Naharro, G., Olivera, E. R., Luengo, J. M. (2005). A Two-component Hydroxylase Involved in the Assimilation of 3-Hydroxyphenyl Acetate in Pseudomonas putida. J. Biol. Chem. 280: 26435-26447 [Abstract] [Full Text]  
• Arias-Barrau, E., Olivera, E. R., Luengo, J. M., Fernandez, C., Galan, B., Garcia, J. L., Diaz, E., Minambres, B. (2004). The Homogentisate Pathway: a Central Catabolic Pathway Involved in the Degradation of L-Phenylalanine, L-Tyrosine, and 3-Hydroxyphenylacetate in Pseudomonas putida. J. Bacteriol. 186: 5062-5077 [Abstract] [Full Text]  
• Galan, B., Garcia, J. L., Prieto, M. A. (2004). The PaaX Repressor, a Link between Penicillin G Acylase and the Phenylacetyl-Coenzyme A Catabolon of Escherichia coli W. J. Bacteriol. 186: 2215-2220 [Abstract] [Full Text]  
• Zeng, L., Jin, S. (2003). aph(3')-IIb, a Gene Encoding an Aminoglycoside-Modifying Enzyme, Is under the Positive Control of Surrogate Regulator HpaA. Antimicrob. Agents Chemother. 47: 3867-3876 [Abstract] [Full Text]  
• Diaz, E., Ferrandez, A., Prieto, M. A., Garcia, J. L. (2001). Biodegradation of Aromatic Compounds by Escherichia coli. Microbiol. Mol. Biol. Rev. 65: 523-569 [Abstract] [Full Text]  
• Xun, L., Sandvik, E. R. (2000). Characterization of 4-Hydroxyphenylacetate 3-Hydroxylase (HpaB) of Escherichia coli as a Reduced Flavin Adenine Dinucleotide-Utilizing Monooxygenase. Appl. Environ. Microbiol. 66: 481-486 [Abstract] [Full Text]  
• Galán, B., Díaz, E., Prieto, M. A., García, J. L. (2000). Functional Analysis of the Small Component of the 4-Hydroxyphenylacetate 3-Monooxygenase of Escherichia coli W: a Prototype of a New Flavin:NAD(P)H Reductase Subfamily. J. Bacteriol. 182: 627-636 [Abstract] [Full Text]  
• Ferrandez, A., Minambres, B., Garcia, B., Olivera, E. R., Luengo, J. M., Garcia, J. L., Diaz, E. (1998). Catabolism of Phenylacetic Acid in Escherichia coli. CHARACTERIZATION OF A NEW AEROBIC HYBRID PATHWAY. J. Biol. Chem. 273: 25974-25986 [Abstract] [Full Text]