This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Goldberg, M B
Right arrow Articles by Sansonetti, P J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goldberg, M B
Right arrow Articles by Sansonetti, P J

 Previous Article  |  Next Article 

J Bacteriol. 1993 April; 175(8): 2189-2196

research-article

Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.

M B Goldberg, O Bârzu, C Parsot and P J Sansonetti

Unité de Pathogénie Microbienne Moléculaire, Institut Pasteur, Paris, France.

ABSTRACT

Shigella flexneri uses elements of the host cell cytoskeleton to move within cells and from cell to cell. IcsA, an S. flexneri protein involved in this movement, was purified and studied in vitro. IcsA bound the radiolabelled ATP analog 3'(2')-O-(4-benzoyl)benzoyl-ATP and hydrolyzed ATP. In addition, the surface localization of IcsA on both extracellular and intracellular shigellae was unipolar. Further, in HeLa cells infected with shigellae, IcsA antiserum labelled the actin tail throughout its length, thereby suggesting that IcsA interacts with elements within the tail. Localization of IcsA within the tail at a distance from the bacterium would require its secretion; we demonstrate here that in vitro IcsA is secreted into the culture supernatant in a cleaved form.

J Bacteriol. 1993 April; 175(8): 2189-2196




This article has been cited by other articles:

  • Janakiraman, A., Fixen, K. R., Gray, A. N., Niki, H., Goldberg, M. B. (2009). A Genome-Scale Proteomic Screen Identifies a Role for DnaK in Chaperoning of Polar Autotransporters in Shigella. J. Bacteriol. 191: 6300-6311 [Abstract] [Full Text]  
  • Wagner, J. K., Heindl, J. E., Gray, A. N., Jain, S., Goldberg, M. B. (2009). Contribution of the Periplasmic Chaperone Skp to Efficient Presentation of the Autotransporter IcsA on the Surface of Shigella flexneri. J. Bacteriol. 191: 815-821 [Abstract] [Full Text]  
  • May, K. L., Morona, R. (2008). Mutagenesis of the Shigella flexneri Autotransporter IcsA Reveals Novel Functional Regions Involved in IcsA Biogenesis and Recruitment of Host Neural Wiscott-Aldrich Syndrome Protein. J. Bacteriol. 190: 4666-4676 [Abstract] [Full Text]  
  • Schroeder, G. N., Hilbi, H. (2008). Molecular Pathogenesis of Shigella spp.: Controlling Host Cell Signaling, Invasion, and Death by Type III Secretion. Clin. Microbiol. Rev. 21: 134-156 [Abstract] [Full Text]  
  • Jain, S., Goldberg, M. B. (2007). Requirement for YaeT in the Outer Membrane Assembly of Autotransporter Proteins. J. Bacteriol. 189: 5393-5398 [Abstract] [Full Text]  
  • Pradel, N., Santini, C.-L., Bernadac, A., Fukumori, Y., Wu, L.-F. (2006). Biogenesis of actin-like bacterial cytoskeletal filaments destined for positioning prokaryotic magnetic organelles. Proc. Natl. Acad. Sci. USA 103: 17485-17489 [Abstract] [Full Text]  
  • Jain, S., van Ulsen, P., Benz, I., Schmidt, M. A., Fernandez, R., Tommassen, J., Goldberg, M. B. (2006). Polar Localization of the Autotransporter Family of Large Bacterial Virulence Proteins. J. Bacteriol. 188: 4841-4850 [Abstract] [Full Text]  
  • Santapaola, D., Del Chierico, F., Petrucca, A., Uzzau, S., Casalino, M., Colonna, B., Sessa, R., Berlutti, F., Nicoletti, M. (2006). Apyrase, the Product of the Virulence Plasmid-Encoded phoN2 (apy) Gene of Shigella flexneri, Is Necessary for Proper Unipolar IcsA Localization and for Efficient Intercellular Spread. J. Bacteriol. 188: 1620-1627 [Abstract] [Full Text]  
  • Zhao, J., Lambowitz, A. M. (2005). Inaugural Article: A bacterial group II intron-encoded reverse transcriptase localizes to cellular poles. Proc. Natl. Acad. Sci. USA 102: 16133-16140 [Abstract] [Full Text]  
  • Burton, E. A., Oliver, T. N., Pendergast, A. M. (2005). Abl Kinases Regulate Actin Comet Tail Elongation via an N-WASP-Dependent Pathway. Mol. Cell. Biol. 25: 8834-8843 [Abstract] [Full Text]  
  • Nilsen, T., Yan, A. W., Gale, G., Goldberg, M. B. (2005). Presence of Multiple Sites Containing Polar Material in Spherical Escherichia coli Cells That Lack MreB. J. Bacteriol. 187: 6187-6196 [Abstract] [Full Text]  
  • Wing, H. J., Goldman, S. R., Ally, S., Goldberg, M. B. (2005). Modulation of an Outer Membrane Protease Contributes to the Virulence Defect of Shigella flexneri Strains Carrying a Mutation in the virK Locus. Infect. Immun. 73: 1217-1220 [Abstract] [Full Text]  
  • Henderson, I. R., Navarro-Garcia, F., Desvaux, M., Fernandez, R. C., Ala'Aldeen, D. (2004). Type V Protein Secretion Pathway: the Autotransporter Story. Microbiol. Mol. Biol. Rev. 68: 692-744 [Abstract] [Full Text]  
  • Janakiraman, A., Goldberg, M. B. (2004). From the Cover: Evidence for polar positional information independent of cell division and nucleoid occlusion. Proc. Natl. Acad. Sci. USA 101: 835-840 [Abstract] [Full Text]  
  • Uruno, T., Liu, J., Li, Y., Smith, N., Zhan, X. (2003). Sequential Interaction of Actin-related Proteins 2 and 3 (Arp2/3) Complex with Neural Wiscott-Aldrich Syndrome Protein (N-WASP) and Cortactin during Branched Actin Filament Network Formation. J. Biol. Chem. 278: 26086-26093 [Abstract] [Full Text]  
  • Shapiro, L., McAdams, H. H., Losick, R. (2002). Generating and Exploiting Polarity in Bacteria. Science 298: 1942-1946 [Abstract] [Full Text]  
  • Goldberg, M. B. (2001). Actin-Based Motility of Intracellular Microbial Pathogens. Microbiol. Mol. Biol. Rev. 65: 595-626 [Abstract] [Full Text]  
  • Suzuki, T., Sasakawa, C. (2001). Molecular Basis of the Intracellular Spreading of Shigella. Infect. Immun. 69: 5959-5966 [Full Text]  
  • Charles, M., Perez, M., Kobil, J. H., Goldberg, M. B. (2001). Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc. Natl. Acad. Sci. USA 10.1073/pnas.171310498v1 [Abstract] [Full Text]  
  • Brandon, L. D., Goldberg, M. B. (2001). Periplasmic Transit and Disulfide Bond Formation of the Autotransported Shigella Protein IcsA. J. Bacteriol. 183: 951-958 [Abstract] [Full Text]  
  • Suzuki, T., Mimuro, H., Miki, H., Takenawa, T., Sasaki, T., Nakanishi, H., Takai, Y., Sasakawa, C. (2000). Rho Family Gtpase Cdc42 Is Essential for the Actin-Based Motility of Shigella in Mammalian Cells. JEM 191: 1905-1920 [Abstract] [Full Text]  
  • Maurer, J., Jose, J., Meyer, T. F. (1999). Characterization of the Essential Transport Function of the AIDA-I Autotransporter and Evidence Supporting Structural Predictions. J. Bacteriol. 181: 7014-7020 [Abstract] [Full Text]  
  • Arondel, J., Singer, M., Matsukawa, A., Zychlinsky, A., Sansonetti, P. J. (1999). Increased Interleukin-1 (IL-1) and Imbalance between IL-1 and IL-1 Receptor Antagonist during Acute Inflammation in Experimental Shigellosis. Infect. Immun. 67: 6056-6066 [Abstract] [Full Text]  
  • Egile, C., Loisel, T. P., Laurent, V., Li, R., Pantaloni, D., Sansonetti, P. J., Carlier, M.-F. (1999). Activation of the Cdc42 Effector N-Wasp by the Shigella flexneri Icsa Protein Promotes Actin Nucleation by Arp2/3 Complex and Bacterial Actin-Based Motility. JCB 146: 1319-1332 [Abstract] [Full Text]  
  • Lindenthal, C., Elsinghorst, E. A. (1999). Identification of a Glycoprotein Produced by Enterotoxigenic Escherichia coli. Infect. Immun. 67: 4084-4091 [Abstract] [Full Text]  
  • Heinzen, R. A., Grieshaber, S. S., Van Kirk, L. S., Devin, C. J. (1999). Dynamics of Actin-Based Movement by Rickettsia rickettsii in Vero Cells. Infect. Immun. 67: 4201-4207 [Abstract] [Full Text]  
  • Losick, R., Shapiro, L. (1999). Changing Views on the Nature of the Bacterial Cell: from Biochemistry to Cytology. J. Bacteriol. 181: 4143-4145 [Full Text]  
  • Schuch, R., Maurelli, A. T. (1999). The Mxi-Spa Type III Secretory Pathway of Shigella flexneri Requires an Outer Membrane Lipoprotein, MxiM, for Invasin Translocation. Infect. Immun. 67: 1982-1991 [Abstract] [Full Text]  
  • Way, S. S., Sallustio, S., Magliozzo, R. S., Goldberg, M. B. (1999). Impact of either Elevated or Decreased Levels of Cytochrome bd Expression on Shigella flexneri Virulence. J. Bacteriol. 181: 1229-1237 [Abstract] [Full Text]  
  • Charles, M., Magdalena, J., Theriot, J. A., Goldberg, M. B. (1999). Functional Analysis of a Rickettsial OmpA Homology Domain of Shigella flexneri IcsA. J. Bacteriol. 181: 869-878 [Abstract] [Full Text]  
  • Blanc-Potard, A.-B., Solomon, F., Kayser, J., Groisman, E. A. (1999). The SPI-3 Pathogenicity Island of Salmonella enterica. J. Bacteriol. 181: 998-1004 [Abstract] [Full Text]  
  • Gouin, E, Gantelet, H, Egile, C, Lasa, I, Ohayon, H, Villiers, V, Gounon, P, Sansonetti, P., Cossart, P (1999). A comparative study of the actin-based motilities of the pathogenic bacteria Listeria monocytogenes, Shigella flexneri and Rickettsia conorii. J. Cell Sci. 112: 1697-1708 [Abstract]  
  • Sandlin, R. C., Maurelli, A. T. (1999). Establishment of Unipolar Localization of IcsA in Shigella flexneri 2a Is Not Dependent on Virulence Plasmid Determinants. Infect. Immun. 67: 350-356 [Abstract] [Full Text]  
  • Hong, M., Gleason, Y., Wyckoff, E. E., Payne, S. M. (1998). Identification of Two Shigella flexneri Chromosomal Loci Involved in Intercellular Spreading. Infect. Immun. 66: 4700-4710 [Abstract] [Full Text]  
  • Yu, J. (1998). Inactivation of DsbA, but Not DsbC and DsbD, Affects the Intracellular Survival and Virulence of Shigella flexneri. Infect. Immun. 66: 3909-3917 [Abstract] [Full Text]  
  • Cersini, A., Salvia, A. M., Bernardini, M. L. (1998). Intracellular Multiplication and Virulence of Shigella flexneri Auxotrophic Mutants. Infect. Immun. 66: 549-557 [Abstract] [Full Text]  
  • Nataro, J. P., Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clin. Microbiol. Rev. 11: 142-201 [Abstract] [Full Text]  
  • Laine, R. O., Zeile, W., Kang, F., Purich, D. L., Southwick, F. S. (1997). Vinculin Proteolysis Unmasks an ActA Homolog for Actin-based Shigella Motility. JCB 138: 1255-1264 [Abstract] [Full Text]  
  • Shapiro, L., Losick, R. (1997). Protein Localization and Cell Fate in Bacteria. Science 276: 712-718 [Abstract] [Full Text]  
  • Meyer, D.H., Mintz, K.P., Fives-Taylor, P.M. (1997). Models of Invasion of Enteric and Periodontal Pathogens Into Epithelial Cells: A Comparative Analysis. CROBM 8: 389-409 [Abstract] [Full Text]  
  • Suzuki, T., Saga, S., Sasakawa, C. (1996). Functional Analysis of Shigella VirG Domains Essential for Interaction with Vinculin and Actin-based Motility. J. Biol. Chem. 271: 21878-21885 [Abstract] [Full Text]  
  • Mimuro, H., Suzuki, T., Suetsugu, S., Miki, H., Takenawa, T., Sasakawa, C. (2000). Profilin Is Required for Sustaining Efficient Intra- and Intercellular Spreading of Shigella flexneri. J. Biol. Chem. 275: 28893-28901 [Abstract] [Full Text]  
  • Charles, M., Perez, M., Kobil, J. H., Goldberg, M. B. (2001). Polar targeting of Shigella virulence factor IcsA in Enterobacteriacae and Vibrio. Proc. Natl. Acad. Sci. USA 98: 9871-9876 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»