This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Benson, A K Articles by Haldenwang, W G Search for Related Content PubMed PubMed Citation Articles by Benson, A K Articles by Haldenwang, W G

research-article

Regulation of sigma B levels and activity in Bacillus subtilis.

Department of Microbiology, University of Texas Health Science Center, San Antonio 78284-7758.

ABSTRACT

The sigB operon of Bacillus subtilis encodes sigma B plus three additional proteins (RsbV, RsbW, and RsbX) that regulate sigma B activity. Using an anti-sigma B monoclonal antibody to monitor the levels of sigma B protein, PSPAC to control the expression of the sigB operon, and a ctc-lacZ reporter system to monitor sigma B activity, we observed that the rsbV and rsbW products control sigma B activity at the ctc promoter independently of their effects on sigma B levels. In contrast, RsbX was found to have no effect on expression of ctc when the sigB operon was controlled by PSPAC. The data are consistent with RsbV and RsbW being regulators of sigma B activity and RsbX acting primarily as a negative regulator of sigB operon expression. Evidence that stationary-phase induction of the sigma B-dependent ctc promoter is accomplished by a reduction in RsbW-dependent inhibition of sigma B activity is also presented. In addition, Western blot (immunoblot) analyses of sigB operon expression demonstrated that sigma B accumulation is coupled to the synthesis of its primary inhibitor (RsbW). This finding is consistent with RsbW and sigma B being present within the cell in equivalent amounts, a circumstance that would permit RsbW to directly influence sigma B activity by a direct protein-protein interaction.

This article has been cited by other articles:

• Haeusser, D. P., Garza, A. C., Buscher, A. Z., Levin, P. A. (2007). The Division Inhibitor EzrA Contains a Seven-Residue Patch Required for Maintaining the Dynamic Nature of the Medial FtsZ Ring. J. Bacteriol. 189: 9001-9010 [Abstract] [Full Text]  
• Hardwick, S. W., Pane-Farre, J., Delumeau, O., Marles-Wright, J., Murray, J. W., Hecker, M., Lewis, R. J. (2007). Structural and Functional Characterization of Partner Switching Regulating the Environmental Stress Response in Bacillus subtilis. J. Biol. Chem. 282: 11562-11572 [Abstract] [Full Text]  
• D'Elia, M. A., Millar, K. E., Beveridge, T. J., Brown, E. D. (2006). Wall Teichoic Acid Polymers Are Dispensable for Cell Viability in Bacillus subtilis. J. Bacteriol. 188: 8313-8316 [Abstract] [Full Text]  
• Delumeau, O., Chen, C.-C., Murray, J. W., Yudkin, M. D., Lewis, R. J. (2006). High-Molecular-Weight Complexes of RsbR and Paralogues in the Environmental Signaling Pathway of Bacillus subtilis. J. Bacteriol. 188: 7885-7892 [Abstract] [Full Text]  
• Miethke, M., Hecker, M., Gerth, U. (2006). Involvement of Bacillus subtilis ClpE in CtsR Degradation and Protein Quality Control. J. Bacteriol. 188: 4610-4619 [Abstract] [Full Text]  
• Auchtung, J. M., Lee, C. A., Grossman, A. D. (2006). Modulation of the ComA-Dependent Quorum Response in Bacillus subtilis by Multiple Rap Proteins and Phr Peptides.. J. Bacteriol. 188: 5273-5285 [Abstract] [Full Text]  
• D'Elia, M. A., Pereira, M. P., Chung, Y. S., Zhao, W., Chau, A., Kenney, T. J., Sulavik, M. C., Black, T. A., Brown, E. D. (2006). Lesions in Teichoic Acid Biosynthesis in Staphylococcus aureus Lead to a Lethal Gain of Function in the Otherwise Dispensable Pathway.. J. Bacteriol. 188: 4183-4189 [Abstract] [Full Text]  
• Zhang, S., Reeves, A., Woodbury, R. L., Haldenwang, W. G. (2005). Coexpression Patterns of {sigma}B Regulators in Bacillus subtilis Affect {sigma}B Inducibility. J. Bacteriol. 187: 8520-8525 [Abstract] [Full Text]  
• Zhang, H., Morikawa, K., Ohta, T., Kato, Y. (2005). In vitro resistance to the CS{alpha}{beta}-type antimicrobial peptide ASABF- is conferred by overexpression of sigma factor sigB in Staphylococcus aureus. J Antimicrob Chemother 55: 686-691 [Abstract] [Full Text]  
• Lee, E.-J., Cho, Y.-H., Kim, H.-S., Ahn, B.-E., Roe, J.-H. (2004). Regulation of {sigma}B by an Anti- and an Anti-Anti-Sigma Factor in Streptomyces coelicolor in Response to Osmotic Stress. J. Bacteriol. 186: 8490-8498 [Abstract] [Full Text]  
• Bhavsar, A. P., Erdman, L. K., Schertzer, J. W., Brown, E. D. (2004). Teichoic Acid Is an Essential Polymer in Bacillus subtilis That Is Functionally Distinct from Teichuronic Acid. J. Bacteriol. 186: 7865-7873 [Abstract] [Full Text]  
• Kuo, S., Zhang, S., Woodbury, R. L., Haldenwang, W. G. (2004). Associations between Bacillus subtilis {sigma}B regulators in cell extracts. Microbiology 150: 4125-4136 [Abstract] [Full Text]  
• Holtmann, G., Brigulla, M., Steil, L., Schutz, A., Barnekow, K., Volker, U., Bremer, E. (2004). RsbV-Independent Induction of the SigB-Dependent General Stress Regulon of Bacillus subtilis during Growth at High Temperature. J. Bacteriol. 186: 6150-6158 [Abstract] [Full Text]  
• Woodbury, R. L., Luo, T., Grant, L., Haldenwang, W. G. (2004). Mutational Analysis of RsbT, an Activator of the Bacillus subtilis Stress Response Transcription Factor, {sigma}B. J. Bacteriol. 186: 2789-2797 [Abstract] [Full Text]  
• McBride, S., Haldenwang, W. G. (2004). Sporulation Phenotype of a Bacillus subtilis Mutant Expressing an Unprocessable but Active {sigma}E Transcription Factor. J. Bacteriol. 186: 1999-2005 [Abstract] [Full Text]  
• Mendez, M. B., Orsaria, L. M., Philippe, V., Pedrido, M. E., Grau, R. R. (2004). Novel Roles of the Master Transcription Factors Spo0A and {sigma}B for Survival and Sporulation of Bacillus subtilis at Low Growth Temperature. J. Bacteriol. 186: 989-1000 [Abstract] [Full Text]  
• Zilhao, R., Serrano, M., Isticato, R., Ricca, E., Moran, C. P. Jr., Henriques, A. O. (2004). Interactions among CotB, CotG, and CotH during Assembly of the Bacillus subtilis Spore Coat. J. Bacteriol. 186: 1110-1119 [Abstract] [Full Text]  
• van Schaik, W., Tempelaars, M. H., Wouters, J. A., de Vos, W. M., Abee, T. (2004). The Alternative Sigma Factor {sigma}B of Bacillus cereus: Response to Stress and Role in Heat Adaptation. J. Bacteriol. 186: 316-325 [Abstract] [Full Text]  
• Zhang, S., Haldenwang, W. G. (2003). RelA Is a Component of the Nutritional Stress Activation Pathway of the Bacillus subtilis Transcription Factor {sigma}B. J. Bacteriol. 185: 5714-5721 [Abstract] [Full Text]  
• Brigulla, M., Hoffmann, T., Krisp, A., Volker, A., Bremer, E., Volker, U. (2003). Chill Induction of the SigB-Dependent General Stress Response in Bacillus subtilis and Its Contribution to Low-Temperature Adaptation. J. Bacteriol. 185: 4305-4314 [Abstract] [Full Text]  
• Lee, J.-C., Stewart, G. C. (2003). Essential Nature of the mreC Determinant of Bacillus subtilis. J. Bacteriol. 185: 4490-4498 [Abstract] [Full Text]  
• Serrano, M., Corte, L., Opdyke, J., Moran, C. P. Jr., Henriques, A. O. (2003). Expression of spoIIIJ in the Prespore Is Sufficient for Activation of {sigma}G and for Sporulation in Bacillus subtilis. J. Bacteriol. 185: 3905-3917 [Abstract] [Full Text]  
• Novak, K. F., Dougherty, B., Pelaez, M. (2001). Actinobacillus actinomycetemcomitans harbours type IV secretion system genes on a plasmid and in the chromosome. Microbiology 147: 3027-3035 [Abstract] [Full Text]  
• Petersohn, A., Brigulla, M., Haas, S., Hoheisel, J. D., Volker, U., Hecker, M. (2001). Global Analysis of the General Stress Response of Bacillus subtilis. J. Bacteriol. 183: 5617-5631 [Abstract] [Full Text]  
• Bischoff, M., Berger-Bächi, B. (2001). Teicoplanin Stress-Selected Mutations Increasing {sigma}B Activity in Staphylococcus aureus. Antimicrob. Agents Chemother. 45: 1714-1720 [Abstract] [Full Text]  
• Zhang, S., Scott, J. M., Haldenwang, W. G. (2001). Loss of Ribosomal Protein L11 Blocks Stress Activation of the Bacillus subtilis Transcription Factor {sigma}B. J. Bacteriol. 183: 2316-2321 [Abstract] [Full Text]  
• Lemon, K. P., Kurtser, I., Grossman, A. D. (2000). Effects of replication termination mutants on chromosome partitioning in Bacillussubtilis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.011506098v1 [Abstract] [Full Text]  
• Bignell, D. R. D., Warawa, J. L., Strap, J. L., Chater, K. F., Leskiw, B. K. (2000). Study of the bldG locus suggests that an anti-anti-sigma factor and an anti-sigma factor may be involved in Streptomyces coelicolor antibiotic production and sporulation. Microbiology 146: 2161-2173 [Abstract] [Full Text]  
• Scott, J. M., Ju, J., Mitchell, T., Haldenwang, W. G. (2000). The Bacillus subtilis GTP Binding Protein Obg and Regulators of the sigma B Stress Response Transcription Factor Cofractionate with Ribosomes. J. Bacteriol. 182: 2771-2777 [Abstract] [Full Text]  
• Scott, J. M., Mitchell, T., Haldenwang, W. G. (2000). Stress Triggers a Process That Limits Activation of the Bacillus subtilis Stress Transcription Factor sigma B. J. Bacteriol. 182: 1452-1456 [Abstract] [Full Text]  
• Ju, J., Mitchell, T., Peters, H. III, Haldenwang, W. G. (1999). Sigma Factor Displacement from RNA Polymerase during Bacillus subtilis Sporulation. J. Bacteriol. 181: 4969-4977 [Abstract] [Full Text]  
• Scott, J. M., Haldenwang, W. G. (1999). Obg, an Essential GTP Binding Protein of Bacillus subtilis, Is Necessary for Stress Activation of Transcription Factor sigma B. J. Bacteriol. 181: 4653-4660 [Abstract] [Full Text]  
• Serrano, M., Zilhão, R., Ricca, E., Ozin, A. J., Moran, C. P. Jr., Henriques, A. O. (1999). A Bacillus subtilis Secreted Protein with a Role in Endospore Coat Assembly and Function. J. Bacteriol. 181: 3632-3643 [Abstract] [Full Text]  
• Mekjian, K. R., Bryan, E. M., Beall, B. W., Moran, C. P. Jr. (1999). Regulation of Hexuronate Utilization in Bacillus subtilis. J. Bacteriol. 181: 426-433 [Abstract] [Full Text]  
• Lemon, K. P., Grossman, A. D. (1998). Localization of Bacterial DNA Polymerase: Evidence for a Factory Model of Replication. Science 282: 1516-1519 [Abstract] [Full Text]  
• Becker, L. A., Çetin, M. S., Hutkins, R. W., Benson, A. K. (1998). Identification of the Gene Encoding the Alternative Sigma Factor sigma B from Listeria monocytogenes and Its Role in Osmotolerance. J. Bacteriol. 180: 4547-4554 [Abstract] [Full Text]  
• Smirnova, N., Scott, J., Voelker, U., Haldenwang, W. G. (1998). Isolation and Characterization of Bacillus subtilis sigB Operon Mutations That Suppress the Loss of the Negative Regulator RsbX. J. Bacteriol. 180: 3671-3680 [Abstract] [Full Text]  
• Henriques, A. O., Melsen, L. R., Moran, C. P. Jr. (1998). Involvement of Superoxide Dismutase in Spore Coat Assembly in Bacillus subtilis. J. Bacteriol. 180: 2285-2291 [Abstract] [Full Text]  
• Britton, R. A., Lin, D. C.-H., Grossman, A. D. (1998). Characterization of a prokaryotic SMC protein involved in chromosome partitioning. Genes Dev. 12: 1254-1259 [Abstract] [Full Text]  
• Ju, J., Luo, T., Haldenwang, W. G. (1998). Forespore Expression and Processing of the SigE Transcription Factor in Wild-Type and Mutant Bacillus subtilis. J. Bacteriol. 180: 1673-1681 [Abstract] [Full Text]  
• Lemon, K. P., Kurtser, I., Grossman, A. D. (2001). Effects of replication termination mutants on chromosome partitioning in Bacillussubtilis. Proc. Natl. Acad. Sci. USA 98: 212-217 [Abstract] [Full Text]