This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McTavish, H Articles by Hooper, A B Search for Related Content PubMed PubMed Citation Articles by McTavish, H Articles by Hooper, A B

 Previous Article  |  Next Article 

J Bacteriol. 1993 April; 175(8): 2436-2444

research-article

Sequence of the gene coding for ammonia monooxygenase in Nitrosomonas europaea.

Department of Genetics and Cell Biology, University of Minnesota, St. Paul 55108.

ABSTRACT

Nitrosomonas europaea, a chemolithotrophic bacterium, was found to contain two copies of the gene coding for the presumed active site polypeptide of ammonia monooxygenase, the 32-kDa acetylene-binding polypeptide. One copy of this gene was cloned, and its complete nucleotide sequence is presented. Immediately downstream of this gene, in the same operon, is the gene for a 40-kDa polypeptide that copurifies with the ammonia monooxygenase acetylene-binding polypeptide. The sequence of the first 692 nucleotides of this structural gene, coding for about two-thirds of the protein, is presented. These sequences are the first sequences of protein-encoding genes from an ammonia-oxidizing autotrophic nitrifying bacterium. The two protein sequences are not homologous with the sequences of any other monooxygenase. From radioactive labelling of ammonia monooxygenase with [14C]acetylene it was determined that there are 23 nmol of ammonia monooxygenase per g of cells. The kcat of ammonia monooxygenase for NH3 in vivo was calculated to be 20 s-1.

This article has been cited by other articles:

• Berube, P. M., Samudrala, R., Stahl, D. A. (2007). Transcription of All amoC Copies Is Associated with Recovery of Nitrosomonas europaea from Ammonia Starvation. J. Bacteriol. 189: 3935-3944 [Abstract] [Full Text]  
• Klotz, M. G., Arp, D. J., Chain, P. S. G., El-Sheikh, A. F., Hauser, L. J., Hommes, N. G., Larimer, F. W., Malfatti, S. A., Norton, J. M., Poret-Peterson, A. T., Vergez, L. M., Ward, B. B. (2006). Complete Genome Sequence of the Marine, Chemolithoautotrophic, Ammonia-Oxidizing Bacterium Nitrosococcus oceani ATCC 19707. Appl. Environ. Microbiol. 72: 6299-6315 [Abstract] [Full Text]  
• Bergmann, D. J., Hooper, A. B., Klotz, M. G. (2005). Structure and Sequence Conservation of hao Cluster Genes of Autotrophic Ammonia-Oxidizing Bacteria: Evidence for Their Evolutionary History. Appl. Environ. Microbiol. 71: 5371-5382 [Abstract] [Full Text]  
• O'Mullan, G. D., Ward, B. B. (2005). Relationship of Temporal and Spatial Variabilities of Ammonia-Oxidizing Bacteria to Nitrification Rates in Monterey Bay, California. Appl. Environ. Microbiol. 71: 697-705 [Abstract] [Full Text]  
• Risgaard-Petersen, N., Nicolaisen, M. H., Revsbech, N. P., Lomstein, B. A. (2004). Competition between Ammonia-Oxidizing Bacteria and Benthic Microalgae. Appl. Environ. Microbiol. 70: 5528-5537 [Abstract] [Full Text]  
• Cole, A. C., Semmens, M. J., LaPara, T. M. (2004). Stratification of Activity and Bacterial Community Structure in Biofilms Grown on Membranes Transferring Oxygen. Appl. Environ. Microbiol. 70: 1982-1989 [Abstract] [Full Text]  
• Koper, T. E., El-Sheikh, A. F., Norton, J. M., Klotz, M. G. (2004). Urease-Encoding Genes in Ammonia-Oxidizing Bacteria. Appl. Environ. Microbiol. 70: 2342-2348 [Abstract] [Full Text]  
• Purkhold, U., Wagner, M., Timmermann, G., Pommerening-Roser, A., Koops, H.-P. (2003). 16S rRNA and amoA-based phylogeny of 12 novel betaproteobacterial ammonia-oxidizing isolates: extension of the dataset and proposal of a new lineage within the nitrosomonads. Int. J. Syst. Evol. Microbiol. 53: 1485-1494 [Abstract] [Full Text]  
• Mintie, A. T., Heichen, R. S., Cromack, K. Jr., Myrold, D. D., Bottomley, P. J. (2003). Ammonia-Oxidizing Bacteria along Meadow-to-Forest Transects in the Oregon Cascade Mountains. Appl. Environ. Microbiol. 69: 3129-3136 [Abstract] [Full Text]  
• Chain, P., Lamerdin, J., Larimer, F., Regala, W., Lao, V., Land, M., Hauser, L., Hooper, A., Klotz, M., Norton, J., Sayavedra-Soto, L., Arciero, D., Hommes, N., Whittaker, M., Arp, D. (2003). Complete Genome Sequence of the Ammonia-Oxidizing Bacterium and Obligate Chemolithoautotroph Nitrosomonas europaea. J. Bacteriol. 185: 2759-2773 [Abstract] [Full Text]  
• Rhodes, L. D., Coady, A. M., Strom, M. S. (2002). Expression of Duplicate msa Genes in the Salmonid Pathogen Renibacteriumsalmoninarum. Appl. Environ. Microbiol. 68: 5480-5487 [Abstract] [Full Text]  
• Webster, G., Embley, T. M., Prosser, J. I. (2002). Grassland Management Regimens Reduce Small-Scale Heterogeneity and Species Diversity of {beta}-Proteobacterial Ammonia Oxidizer Populations. Appl. Environ. Microbiol. 68: 20-30 [Abstract] [Full Text]  
• Dionisi, H. M., Layton, A. C., Harms, G., Gregory, I. R., Robinson, K. G., Sayler, G. S. (2002). Quantification of Nitrosomonas oligotropha-Like Ammonia-Oxidizing Bacteria and Nitrospira spp. from Full-Scale Wastewater Treatment Plants by Competitive PCR. Appl. Environ. Microbiol. 68: 245-253 [Abstract] [Full Text]  
• Baxter, N. J., Scanlan, J., De Marco, P., Wood, A. P., Murrell, J. C. (2002). Duplicate Copies of Genes Encoding Methanesulfonate Monooxygenase in Marinosulfonomonas methylotropha Strain TR3 and Detection of Methanesulfonate Utilizers in the Environment. Appl. Environ. Microbiol. 68: 289-296 [Abstract] [Full Text]  
• Hermansson, A., Lindgren, P.-E. (2001). Quantification of Ammonia-Oxidizing Bacteria in Arable Soil by Real-Time PCR. Appl. Environ. Microbiol. 67: 972-976 [Abstract] [Full Text]  
• Hommes, N. G., Sayavedra-Soto, L. A., Arp, D. J. (2001). Transcript Analysis of Multiple Copies of amo (Encoding Ammonia Monooxygenase) and hao (Encoding Hydroxylamine Oxidoreductase) in Nitrosomonas europaea. J. Bacteriol. 183: 1096-1100 [Abstract] [Full Text]  
• Pinck, C., Coeur, C., Potier, P., Bock, E. (2001). Polyclonal Antibodies Recognizing the AmoB Protein of Ammonia Oxidizers of the {beta}-Subclass of the Class Proteobacteria. Appl. Environ. Microbiol. 67: 118-124 [Abstract] [Full Text]  
• Purkhold, U., Pommerening-Röser, A., Juretschko, S., Schmid, M. C., Koops, H.-P., Wagner, M. (2000). Phylogeny of All Recognized Species of Ammonia Oxidizers Based on Comparative 16S rRNA and amoA Sequence Analysis: Implications for Molecular Diversity Surveys. Appl. Environ. Microbiol. 66: 5368-5382 [Abstract] [Full Text]  
• Gilbert, B., McDonald, I. R., Finch, R., Stafford, G. P., Nielsen, A. K., Murrell, J. C. (2000). Molecular Analysis of the pmo (Particulate Methane Monooxygenase) Operons from Two Type II Methanotrophs. Appl. Environ. Microbiol. 66: 966-975 [Abstract] [Full Text]  
• Hirota, R., Yamagata, A., Kato, J., Kuroda, A., Ikeda, T., Takiguchi, N., Ohtake, H. (2000). Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11. J. Bacteriol. 182: 825-828 [Abstract] [Full Text]  
• Whitby, C. B., Saunders, J. R., Rodriguez, J., Pickup, R. W., McCarthy, A. (1999). Phylogenetic Differentiation of Two Closely Related Nitrosomonas spp. That Inhabit Different Sediment Environments in an Oligotrophic Freshwater Lake. Appl. Environ. Microbiol. 65: 4855-4862 [Abstract] [Full Text]  
• Costello, A. M., Lidstrom, M. E. (1999). Molecular Characterization of Functional and Phylogenetic Genes from Natural Populations of Methanotrophs in Lake Sediments. Appl. Environ. Microbiol. 65: 5066-5074 [Abstract] [Full Text]  
• Mendum, T. A., Sockett, R. E., Hirsch, P. R. (1999). Use of Molecular and Isotopic Techniques To Monitor the Response of Autotrophic Ammonia-Oxidizing Populations of the beta Subdivision of the Class Proteobacteria in Arable Soils to Nitrogen Fertilizer. Appl. Environ. Microbiol. 65: 4155-4162 [Abstract] [Full Text]  
• Yamagata, A., Kato, J., Hirota, R., Kuroda, A., Ikeda, T., Takiguchi, N., Ohtake, H. (1999). Isolation and Characterization of Two Cryptic Plasmids in the Ammonia-Oxidizing Bacterium Nitrosomonas sp. Strain ENI-11. J. Bacteriol. 181: 3375-3381 [Abstract] [Full Text]  
• Stephen, J. R., Chang, Y.-J., Macnaughton, S. J., Kowalchuk, G. A., Leung, K. T., Flemming, C. A., White, D. C. (1999). Effect of Toxic Metals on Indigenous Soil beta -Subgroup Proteobacterium Ammonia Oxidizer Community Structure and Protection against Toxicity by Inoculated Metal-Resistant Bacteria. Appl. Environ. Microbiol. 65: 95-101 [Abstract] [Full Text]  
• Bergmann, D. J., Zahn, J. A., Hooper, A. B., DiSpirito, A. A. (1998). Cytochrome P460 Genes from the Methanotroph Methylococcus capsulatus Bath. J. Bacteriol. 180: 6440-6445 [Abstract] [Full Text]  
• Hastings, R. C., Saunders, J. R., Hall, G. H., Pickup, R. W., McCarthy, A. J. (1998). Application of Molecular Biological Techniques to a Seasonal Study of Ammonia Oxidation in a Eutrophic Freshwater Lake. Appl. Environ. Microbiol. 64: 3674-3682 [Abstract] [Full Text]  
• Juretschko, S., Timmermann, G., Schmid, M., Schleifer, K.-H., Pommerening-Röser, A., Koops, H.-P., Wagner, M. (1998). Combined Molecular and Conventional Analyses of Nitrifying Bacterium Diversity in Activated Sludge: Nitrosococcus mobilis and Nitrospira-Like Bacteria as Dominant Populations. Appl. Environ. Microbiol. 64: 3042-3051 [Abstract] [Full Text]  
• Holben, W. E., Noto, K., Sumino, T., Suwa, Y. (1998). Molecular Analysis of Bacterial Communities in a Three-Compartment Granular Activated Sludge System Indicates Community-Level Control by Incompatible Nitrification Processes. Appl. Environ. Microbiol. 64: 2528-2532 [Abstract] [Full Text]  
• Hommes, N. G., Sayavedra-Soto, L. A., Arp, D. J. (1998). Mutagenesis and Expression of amo, Which Codes for Ammonia Monooxygenase in Nitrosomonas europaea. J. Bacteriol. 180: 3353-3359 [Abstract] [Full Text]  
• Nguyen, H.-H. T., Elliott, S. J., Yip, J. H.-K., Chan, S. I. (1998). The Particulate Methane Monooxygenase from Methylococcus capsulatus (Bath) Is a Novel Copper-containing Three-subunit Enzyme. ISOLATION AND CHARACTERIZATION. J. Biol. Chem. 273: 7957-7966 [Abstract] [Full Text]