This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Schön, U Articles by Schumann, W Search for Related Content PubMed PubMed Citation Articles by Schön, U Articles by Schumann, W

research-article

Molecular cloning, sequencing, and transcriptional analysis of the groESL operon from Bacillus stearothermophilus.

Lehrstuhl für Genetik, Universität Bayreuth, Germany.

ABSTRACT

Using a gene probe of the Bacillus subtilis groEL gene, a 7.3-kb HindIII fragment of chromosomal DNA of Bacillus stearothermophilus was cloned. Sequencing of 2,309 bp led to the detection of two open reading frames in the order groES groEL. Primer extension studies revealed one potential transcription start site preceding the groESL operon, which was activated upon temperature upshift. Northern blot (RNA) analysis resolved two mRNA species with lengths of 2.2 and 1.5 kb; RNA slot-blot experiments revealed an at least 10-fold increase in the amount of specific mRNA from 0 to 7 min postinduction followed by a decrease. The 9-bp inverted repeat characteristic of many gram-positive bacteria was found within the 5' leader region of the mRNA. The groESL operon of B. stearothermophilus could complement E. coli groES(Ts) and groEL(Ts) mutants for growth at high temperature and for propagation of phage lambda.

This article has been cited by other articles:

• Kuchanny-Ardigo, D., Lipinska, B. (2003). Cloning and characterization of the groE heat-shock operon of the marine bacterium Vibrio harveyi. Microbiology 149: 1483-1492 [Abstract] [Full Text]  
• Minder, A. C., Fischer, H.-M., Hennecke, H., Narberhaus, F. (2000). Role of HrcA and CIRCE in the Heat Shock Regulatory Network of Bradyrhizobium japonicum. J. Bacteriol. 182: 14-22 [Abstract] [Full Text]