This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Orser, C S Articles by Hargis, M Search for Related Content PubMed PubMed Citation Articles by Orser, C S Articles by Hargis, M

research-article

Characterization of a Flavobacterium glutathione S-transferase gene involved reductive dechlorination.

Department of Bacteriology and Biochemistry, University of Idaho, Moscow 83843.

ABSTRACT

The gene pcpC, encoding tetrachloro-p-hydroquinone (TeCH) reductive dehalogenase, was cloned from Flavobacterium sp. strain ATCC 39723 and sequenced. The gene was identified by hybridization with a degenerate oligonucleotide designed from the N-terminal sequence of the purified protein. An open reading frame of 747 nucleotides was found, which predicts a translational product of 248 amino acids having a molecular weight of 28,263, which agrees favorably with the sodium dodecyl sulfate-polyacrylamide gel electrophoresis-determined molecular weight of 30,000 reported for the purified protein. The predicted translational product of pcpC matched the N-terminal sequence of the purified protein exactly. From the nucleotide sequence, the protein appears to have a processed formylmethionyl. An Escherichia coli pcpC overexpression clone was shown to produce dichlorohydroquinone and trichlorohydroquinone from TeCH. Protein data base searches grouped the predicted translational sequence of pcpC with two previously reported plant glutathione S-transferases but less significantly with any of the mammalian glutathione S-transferases or the glutathione-utilizing, hydrolytic dechlorinating enzyme from Methylobacterium sp. strain DM4.

This article has been cited by other articles:

• Wackett, L. P. (2004). Evolution of Enzymes for the Metabolism of New Chemical Inputs into the Environment. J. Biol. Chem. 279: 41259-41262 [Full Text]  
• Dai, M., Copley, S. D. (2004). Genome Shuffling Improves Degradation of the Anthropogenic Pesticide Pentachlorophenol by Sphingobium chlorophenolicum ATCC 39723. Appl. Environ. Microbiol. 70: 2391-2397 [Abstract] [Full Text]  
• Marco, A., Cuesta, A., Pedrola, L., Palau, F., Marin, I. (2004). Evolutionary and Structural Analyses of GDAP1, Involved in Charcot-Marie-Tooth Disease, Characterize a Novel Class of Glutathione Transferase-Related Genes. Mol Biol Evol 21: 176-187 [Abstract] [Full Text]  
• Dai, M., Rogers, J. B., Warner, J. R., Copley, S. D. (2003). A Previously Unrecognized Step in Pentachlorophenol Degradation in Sphingobium chlorophenolicum Is Catalyzed by Tetrachlorobenzoquinone Reductase (PcpD). J. Bacteriol. 185: 302-310 [Abstract] [Full Text]  
• Cai, M., Xun, L. (2002). Organization and Regulation of Pentachlorophenol-Degrading Genes in Sphingobium chlorophenolicum ATCC 39723. J. Bacteriol. 184: 4672-4680 [Abstract] [Full Text]  
• Lohmeier-Vogel, E. M., Leung, K. T., Lee, H., Trevors, J. T., Vogel, H. J. (2001). Phosphorus-31 Nuclear Magnetic Resonance Study of the Effect of Pentachlorophenol (PCP) on the Physiologies of PCP-Degrading Microorganisms. Appl. Environ. Microbiol. 67: 3549-3556 [Abstract] [Full Text]  
• Werwath, J., Arfmann, H.-A., Pieper, D. H., Timmis, K. N., Wittich, R.-M. (1998). Biochemical and Genetic Characterization of a Gentisate 1,2-Dioxygenase from Sphingomonas sp. Strain RW5. J. Bacteriol. 180: 4171-4176 [Abstract] [Full Text]