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J Bacteriol. 1994 January; 176(1): 137-142

research-article

Isolation of suppressors of temperature-sensitive folding mutations.

Department of Microbiology, University of Tennessee, Knoxville 37996.

ABSTRACT

Mutations in the tailspike gene (gene 9) of Salmonella typhimurium phage P22 have been used to identify amino acid interactions during the folding of a polypeptide chain. Since temperature-sensitive folding (tsf) mutations cause folding defects in the P22 tailspike polypeptide chain, it is likely that mutants derived from these and correcting the original tsf defects (second-site intragenic suppressors) identify interactions during the folding pathway. We report the isolation and identification of second-site revertants to tsf mutants.

This article has been cited by other articles:

• Aramli, L. A., Teschke, C. M. (1999). Single Amino Acid Substitutions Globally Suppress the Folding Defects of Temperature-sensitive Folding Mutants of Phage P22 Coat Protein. J. Biol. Chem. 274: 22217-22224 [Abstract] [Full Text]