This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Morona, J K Articles by Paton, J C Search for Related Content PubMed PubMed Citation Articles by Morona, J K Articles by Paton, J C

 Previous Article  |  Next Article 

J Bacteriol. 1994 July; 176(14): 4437-4443

research-article

Isolation, characterization, and nucleotide sequence of IS1202, an insertion sequence of Streptococcus pneumoniae.

Department of Microbiology, Women's and Children's Hospital, North Adelaide, Australia.

ABSTRACT

A comparative hybridization protocol was used to isolate a small segment of DNA present in the Streptococcus pneumoniae type 19F strain SSZ but absent from strain Rx1, a nonencapsulated derivative of the type 2 strain D39. This segment of DNA is a 1,747-bp insertion sequence, designated IS1202, flanked by 23-bp imperfect inverted repeats and containing a single open reading frame sufficient to encode a 54.4-kDa polypeptide. A 27-bp target sequence is duplicated at either end of the element. IS1202 is not related to any of the currently known insertion elements and is the first reported for S. pneumoniae. Although found predominantly in type 19F strains in up to five copies, it has also been shown to be present in the chromosomes of pneumococci belonging to other serotypes. One of the four IS1202 copies in the encapsulated strain SSZ is located 1,009 bp downstream of the dexB gene, and transformation studies reveal that it is also closely linked to the type 19F capsular polysaccharide synthesis (cps) locus.

This article has been cited by other articles:

• Allegrucci, M., Sauer, K. (2008). Formation of Streptococcus pneumoniae Non-Phase-Variable Colony Variants Is Due to Increased Mutation Frequency Present under Biofilm Growth Conditions. J. Bacteriol. 190: 6330-6339 [Abstract] [Full Text]  
• Bender, M. H., Cartee, R. T., Yother, J. (2003). Positive Correlation between Tyrosine Phosphorylation of CpsD and Capsular Polysaccharide Production in Streptococcus pneumoniae. J. Bacteriol. 185: 6057-6066 [Abstract] [Full Text]  
• Morona, J. K., Morona, R., Miller, D. C., Paton, J. C. (2003). Mutational Analysis of the Carboxy-Terminal (YGX)4 Repeat Domain of CpsD, an Autophosphorylating Tyrosine Kinase Required for Capsule Biosynthesis in Streptococcus pneumoniae. J. Bacteriol. 185: 3009-3019 [Abstract] [Full Text]  
• Morona, J. K., Morona, R., Miller, D. C., Paton, J. C. (2002). Streptococcus pneumoniae Capsule Biosynthesis Protein CpsB Is a Novel Manganese-Dependent Phosphotyrosine-Protein Phosphatase. J. Bacteriol. 184: 577-583 [Abstract] [Full Text]  
• Hoskins, J., Alborn, W. E. Jr., Arnold, J., Blaszczak, L. C., Burgett, S., DeHoff, B. S., Estrem, S. T., Fritz, L., Fu, D.-J., Fuller, W., Geringer, C., Gilmour, R., Glass, J. S., Khoja, H., Kraft, A. R., Lagace, R. E., LeBlanc, D. J., Lee, L. N., Lefkowitz, E. J., Lu, J., Matsushima, P., McAhren, S. M., McHenney, M., McLeaster, K., Mundy, C. W., Nicas, T. I., Norris, F. H., O'Gara, M., Peery, R. B., Robertson, G. T., Rockey, P., Sun, P.-M., Winkler, M. E., Yang, Y., Young-Bellido, M., Zhao, G., Zook, C. A., Baltz, R. H., Jaskunas, S. R., Rosteck, P. R. Jr., Skatrud, P. L., Glass, J. I. (2001). Genome of the Bacterium Streptococcus pneumoniae Strain R6. J. Bacteriol. 183: 5709-5717 [Abstract] [Full Text]  
• Morona, J. K., Morona, R., Paton, J. C. (1999). Comparative Genetics of Capsular Polysaccharide Biosynthesis in Streptococcus pneumoniae Types Belonging to Serogroup 19. J. Bacteriol. 181: 5355-5364 [Abstract] [Full Text]  
• Ramirez, M., Tomasz, A. (1998). Molecular Characterization of the Complete 23F Capsular Polysaccharide Locus of Streptococcus pneumoniae. J. Bacteriol. 180: 5273-5278 [Abstract] [Full Text]  
• Muñoz, R., López, R., García, E. (1998). Characterization of IS1515, a Functional Insertion Sequence in Streptococcus pneumoniae. J. Bacteriol. 180: 1381-1388 [Abstract] [Full Text]