This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kong, D Articles by Masker, W Search for Related Content PubMed PubMed Citation Articles by Kong, D Articles by Masker, W

 Previous Article  |  Next Article 

J Bacteriol. 1994 October; 176(19): 5904-5911

research-article

Deletion between direct repeats in T7 DNA stimulated by double-strand breaks.

Department of Biochemistry and Molecular Biology, Temple University School of Medicine, Philadelphia, Pennsylvania 19140.

ABSTRACT

An in vitro system based on extracts of Escherichia coli infected with bacteriophage T7 was used to study genetic deletions between directly repeated sequences. The frequency of deletion was highest under conditions in which the DNA was actively replicating. Deletion frequency increased markedly with the length of the direct repeat both in vitro and in vivo. When a T7 gene was interrupted by 93 bp of nonsense sequence flanked by 20-bp direct repeats, the region between the repeats was deleted in about 1 out of every 1,600 genomes during each round of replication. Very similar values were found for deletion frequency in vivo and in vitro. The deletion frequency was essentially unaffected by a recA mutation in the host. When a double-strand break was placed between the repeats, repair of this strand break was often accompanied by the deletion of the DNA between the direct repeats, suggesting that break rejoining could contribute to deletion during in vitro DNA replication.

This article has been cited by other articles:

• Mashimo, K., Kawata, M., Yamamoto, K. (2003). Roles of the RecJ and RecQ proteins in spontaneous formation of deletion mutations in the Escherichia coli K12 endogenous tonB gene. Mutagenesis 18: 355-363 [Abstract] [Full Text]  
• Puopolo, K. M., Hollingshead, S. K., Carey, V. J., Madoff, L. C. (2001). Tandem Repeat Deletion in the Alpha C Protein of Group B Streptococcus Is recA Independent. Infect. Immun. 69: 5037-5045 [Abstract] [Full Text]  
• Pajunen, M. I., Kiljunen, S. J., Söderholm, M. E.-L., Skurnik, M. (2001). Complete Genomic Sequence of the Lytic Bacteriophage {phi}YeO3-12 of Yersinia enterocolitica Serotype O:3. J. Bacteriol. 183: 1928-1937 [Abstract] [Full Text]  
• Lai, Y.-T., Masker, W. (2000). Visualization of Repair of Double-Strand Breaks in the Bacteriophage T7 Genome without Normal DNA Replication. J. Bacteriol. 182: 327-336 [Abstract] [Full Text]  
• Lai, Y.-T., Masker, W. (1998). In Vitro Repair of Gaps in Bacteriophage T7 DNA. J. Bacteriol. 180: 6193-6202 [Abstract] [Full Text]  
• Kong, D., Richardson, C. C. (1998). Role of the Acidic Carboxyl-terminal Domain of the Single-stranded DNA-binding Protein of Bacteriophage T7 in Specific Protein-Protein Interactions. J. Biol. Chem. 273: 6556-6564 [Abstract] [Full Text]  
• Kong, D., Griffith, J. D., Richardson, C. C. (1997). Gene 4 helicase of bacteriophage T7 mediates strand transfer through pyrimidine dimers, mismatches, and nonhomologous regions. Proc. Natl. Acad. Sci. USA 94: 2987-2992 [Abstract] [Full Text]