This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Sarker, R I Articles by Tsuchiya, T Search for Related Content PubMed PubMed Citation Articles by Sarker, R I Articles by Tsuchiya, T

 Previous Article  |  Next Article 

J Bacteriol. 1994 December; 176(23): 7378-7382

research-article

Characterization of a glucose transport system in Vibrio parahaemolyticus.

Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Japan.

ABSTRACT

Cells of a glucose-PTS (phosphoenolpyruvate:carbohydrate phosphotransferase system)-negative mutant of Vibrio parahaemolyticus transport D-glucose in the presence of Na+. Maximum stimulation of D-glucose transport was observed at 40 mM NaCl, and Na+ could be replaced partially with Li+. Addition of D-glucose to the cell suspension under anaerobic conditions elicited Na+ uptake. Thus, we conclude that glucose is transported by a Na+/glucose symport mechanism. Calculated Vmax and Km values for the Na(+)-dependent D-glucose transport were 15 nmol/min/mg of protein and 0.57 mM, respectively, when NaCl was added at 40 mM. Na+ lowered the Km value without affecting the Vmax value. D-Glucose was the best substrate for this transport system, followed by galactose, alpha-D-fucose, and methyl-alpha-glucoside, judging from the inhibition pattern of the glucose transport. D-Glucose itself partly repressed the transport system when cells were grown in its presence.

This article has been cited by other articles:

• Morita, Y., Kataoka, A., Shiota, S., Mizushima, T., Tsuchiya, T. (2000). NorM of Vibrio parahaemolyticus Is an Na+-Driven Multidrug Efflux Pump. J. Bacteriol. 182: 6694-6697 [Abstract] [Full Text]  
• Button, D. K. (1998). Nutrient Uptake by Microorganisms according to Kinetic Parameters from Theory as Related to Cytoarchitecture. Microbiol. Mol. Biol. Rev. 62: 636-645 [Abstract] [Full Text]  
• Xie, Z., Turk, E., Wright, E. M. (2000). Characterization of the Vibrio parahaemolyticus Na+/Glucose Cotransporter. A BACTERIAL MEMBER OF THE SODIUM/GLUCOSE TRANSPORTER (SGLT) FAMILY. J. Biol. Chem. 275: 25959-25964 [Abstract] [Full Text]