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J Bacteriol. 1994 December; 176(24): 7740-7743

research-article

Serine residue 45 of nodulation protein NodF from Rhizobium leguminosarum bv. viciae is essential for its biological function.

T Ritsema, O Geiger, P van Dillewijn, B J Lugtenberg and H P Spaink

Clusius Laboratory, Institute of Molecular Plant Sciences, Leiden University, The Netherlands.

ABSTRACT

A system for testing the role of the Rhizobium nodF gene in the production of host-specific lipochitin oligosaccharides and in nodulation was developed. We show that a mutant nodF gene, in which the codon for serine residue 45 was changed to that for threonine, still expresses NodF, which, however, is no longer functional.


J Bacteriol. 1994 December; 176(24): 7740-7743




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