This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pogliano, K J Articles by Beckwith, J Search for Related Content PubMed PubMed Citation Articles by Pogliano, K J Articles by Beckwith, J

 Previous Article  |  Next Article 

J Bacteriol. 1994 February; 176(3): 804-814

research-article

Genetic and molecular characterization of the Escherichia coli secD operon and its products.

Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.

ABSTRACT

The secD operon of Escherichia coli is required for the efficient export of proteins. We have characterized this operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports. An analysis of yajC mutations constructed in vitro and recombined onto the chromosome indicates that yajC is neither essential nor a sec gene. The secD operon is not induced in response to either secretion defects or temperature changes. TnphoA fusions have been used to analyze the topology of SecD in the inner membrane; the protein contains six transmembrane stretches and a large periplasmic domain. TnphoA fusions to SecD and SecF have also been recombined onto the chromosome and used to determine the level of these proteins within the cell. Our results indicate that there are fewer than 30 SecD and SecF molecules per cell.

This article has been cited by other articles:

• Hand, N. J., Klein, R., Laskewitz, A., Pohlschroder, M. (2006). Archaeal and Bacterial SecD and SecF Homologs Exhibit Striking Structural and Functional Conservation. J. Bacteriol. 188: 1251-1259 [Abstract] [Full Text]  
• Nouwen, N., Driessen, A. J. M. (2005). Inactivation of Protein Translocation by Cold-Sensitive Mutations in the yajC-secDF Operon. J. Bacteriol. 187: 6852-6855 [Abstract] [Full Text]  
• Woodson, J. D., Reynolds, A. A., Escalante-Semerena, J. C. (2005). ABC Transporter for Corrinoids in Halobacterium sp. Strain NRC-1. J. Bacteriol. 187: 5901-5909 [Abstract] [Full Text]  
• Rahman, M. S., Simser, J. A., Macaluso, K. R., Azad, A. F. (2005). Functional analysis of secA homologues from rickettsiae. Microbiology 151: 589-596 [Abstract] [Full Text]  
• Drew, D., Sjostrand, D., Nilsson, J., Urbig, T., Chin, C.-n., de Gier, J.-W., von Heijne, G. (2002). Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. USA 10.1073/pnas.052018199v1 [Abstract] [Full Text]  
• Vitikainen, M., Pummi, T., Airaksinen, U., Wahlström, E., Wu, H., Sarvas, M., Kontinen, V. P. (2001). Quantitation of the Capacity of the Secretion Apparatus and Requirement for PrsA in Growth and Secretion of {alpha}-Amylase in Bacillus subtilis. J. Bacteriol. 183: 1881-1890 [Abstract] [Full Text]  
• Esberg, B., Leung, H.-C. E., Tsui, H.-C. T., Björk, G. R., Winkler, M. E. (1999). Identification of the miaB Gene, Involved in Methylthiolation of Isopentenylated A37 Derivatives in the tRNA of Salmonella typhimurium and Escherichia coli. J. Bacteriol. 181: 7256-7265 [Abstract] [Full Text]  
• Vemulapalli, R., Duncan, A. J., Boyle, S. M., Sriranganathan, N., Toth, T. E., Schurig, G. G. (1998). Cloning and Sequencing of yajC and secD Homologs of Brucella abortus and Demonstration of Immune Responses to YajC in Mice Vaccinated with B. abortus RB51. Infect. Immun. 66: 5684-5691 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]  
• Bolhuis, A., Broekhuizen, C. P., Sorokin, A., van Roosmalen, M. L., Venema, G., Bron, S., Quax, W. J., van Dijl, J. M. (1998). SecDF of Bacillus subtilis, a Molecular Siamese Twin Required for the Efficient Secretion of Proteins. J. Biol. Chem. 273: 21217-21224 [Abstract] [Full Text]  
• Wickner, W., Leonard, M. R. (1996). Escherichia coli Preprotein Translocase. J. Biol. Chem. 271: 29514-29516 [Full Text]  
• Drew, D., Sjostrand, D., Nilsson, J., Urbig, T., Chin, C.-n., de Gier, J.-W., von Heijne, G. (2002). Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis. Proc. Natl. Acad. Sci. USA 99: 2690-2695 [Abstract] [Full Text]