This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shingler, V Articles by Moore, T Search for Related Content PubMed PubMed Citation Articles by Shingler, V Articles by Moore, T

Next Article 

J Bacteriol. 1994 March; 176(6): 1555-1560

research-article

Sensing of aromatic compounds by the DmpR transcriptional activator of phenol-catabolizing Pseudomonas sp. strain CF600.

Department of Cell and Molecular Biology, University of Umeå, Sweden.

ABSTRACT

The dmp operon of the pVI150 catabolic plasmid of Pseudomonas sp. strain CF600 encodes the enzymes involved in the catabolism of phenol and methylphenols. The regulator of this dmp pathway, DmpR, is a member of the NtrC family of transcriptional activators and controls transcription of the dmp operon in response to aromatic effector compounds (V. Shingler, M. Bartilson, and T. Moore, J. Bacteriol. 175:1596-1604, 1993). Using a lux gene fusion reporter system, in which the DmpR-regulated operon promoter controls the expression of luciferase activity, we have shown in the study reported here that DmpR is activated by, but responds differentially to, the presence of a wide range of aromatic compounds. In many microbial regulatory systems, including some members of the NtrC family, the response to environmental fluctuations involves information transfer from surface sensory proteins to transcriptional regulators. However, DmpR-mediated activation of phenol metabolism in response to aromatic compounds occurs in the absence of a specific sensory protein. We used hybrids between DmpR and XylR, a structurally related regulator of toluene and xylene metabolism, to demonstrate that it is the amino-terminal domains of these regulators that determine the specificity of transcriptional activation. The results suggest that it is the direct interaction of aromatic compounds with the DmpR and XylR proteins that regulates their transcriptional promoting activity.

This article has been cited by other articles:

• Piao, Z., Sze, C. C., Barysheva, O., Iida, K.-i., Yoshida, S.-i. (2006). Temperature-Regulated Formation of Mycelial Mat-Like Biofilms by Legionella pneumophila. Appl. Environ. Microbiol. 72: 1613-1622 [Abstract] [Full Text]  
• Schmeling, S., Narmandakh, A., Schmitt, O., Gad'on, N., Schuhle, K., Fuchs, G. (2004). Phenylphosphate Synthase: a New Phosphotransferase Catalyzing the First Step in Anaerobic Phenol Metabolism in Thauera aromatica. J. Bacteriol. 186: 8044-8057 [Abstract] [Full Text]  
• Suar, M., van der Meer, J. R., Lawlor, K., Holliger, C., Lal, R. (2004). Dynamics of Multiple lin Gene Expression in Sphingomonas paucimobilis B90A in Response to Different Hexachlorocyclohexane Isomers. Appl. Environ. Microbiol. 70: 6650-6656 [Abstract] [Full Text]  
• Solera, D., Arenghi, F. L. G., Woelk, T., Galli, E., Barbieri, P. (2004). TouR-Mediated Effector-Independent Growth Phase-Dependent Activation of the {sigma}54 Ptou Promoter of Pseudomonas stutzeri OX1. J. Bacteriol. 186: 7353-7363 [Abstract] [Full Text]  
• Tropel, D., van der Meer, J. R. (2004). Bacterial Transcriptional Regulators for Degradation Pathways of Aromatic Compounds. Microbiol. Mol. Biol. Rev. 68: 474-500 [Abstract] [Full Text]  
• Jeong, J. J., Kim, J. H., Kim, C.-K., Hwang, I., Lee, K. (2003). 3- and 4-alkylphenol degradation pathway in Pseudomonas sp. strain KL28: genetic organization of the lap gene cluster and substrate specificities of phenol hydroxylase and catechol 2,3-dioxygenase. Microbiology 149: 3265-3277 [Abstract] [Full Text]  
• Teramoto, M., Ohnishi, K., Harayama, S., Watanabe, K. (2002). An AraC/XylS Family Member at a High Level in a Hierarchy of Regulators for Phenol-Metabolizing Enzymes in Comamonas testosteroni R5. J. Bacteriol. 184: 3941-3946 [Abstract] [Full Text]  
• Sze, C. C., Bernardo, L. M. D., Shingler, V. (2002). Integration of Global Regulation of Two Aromatic-Responsive {sigma}54-Dependent Systems: a Common Phenotype by Different Mechanisms. J. Bacteriol. 184: 760-770 [Abstract] [Full Text]  
• Teramoto, M., Harayama, S., Watanabe, K. (2001). PhcS Represses Gratuitous Expression of Phenol-Metabolizing Enzymes in Comamonas testosteroni R5. J. Bacteriol. 183: 4227-4234 [Abstract] [Full Text]  
• Sze, C. C., Laurie, A. D., Shingler, V. (2001). In Vivo and In Vitro Effects of Integration Host Factor at the DmpR-Regulated {sigma}54-Dependent Po Promoter. J. Bacteriol. 183: 2842-2851 [Abstract] [Full Text]  
• Sarand, I., Skärfstad, E., Forsman, M., Romantschuk, M., Shingler, V. (2001). Role of the DmpR-Mediated Regulatory Circuit in Bacterial Biodegradation Properties in Methylphenol-Amended Soils. Appl. Environ. Microbiol. 67: 162-171 [Abstract] [Full Text]  
• Jones, R. M., Williams, P. A. (2001). areCBA Is an Operon in Acinetobacter sp. Strain ADP1 and Is Controlled by AreR, a {sigma}54-Dependent Regulator. J. Bacteriol. 183: 405-409 [Abstract] [Full Text]  
• Breinig, S., Schiltz, E., Fuchs, G. (2000). Genes Involved in Anaerobic Metabolism of Phenol in the Bacterium Thauera aromatica. J. Bacteriol. 182: 5849-5863 [Abstract] [Full Text]  
• Skärfstad, E., O'Neill, E., Garmendia, J., Shingler, V. (2000). Identification of an Effector Specificity Subregion within the Aromatic-Responsive Regulators DmpR and XylR by DNA Shuffling. J. Bacteriol. 182: 3008-3016 [Abstract] [Full Text]  
• Jaspers, M. C. M., Suske, W. A., Schmid, A., Goslings, D. A. M., Kohler, H.-P. E., van der Meer, J. R. (2000). HbpR, a New Member of the XylR/DmpR Subclass within the NtrC Family of Bacterial Transcriptional Activators, Regulates Expression of 2-Hydroxybiphenyl Metabolism in Pseudomonas azelaica HBP1. J. Bacteriol. 182: 405-417 [Abstract] [Full Text]  
• Wise, A. A., Kuske, C. R. (2000). Generation of Novel Bacterial Regulatory Proteins That Detect Priority Pollutant Phenols. Appl. Environ. Microbiol. 66: 163-169 [Abstract] [Full Text]  
• O'Neill, E., Sze, C. C., Shingler, V. (1999). Novel Effector Control through Modulation of a Preexisting Binding Site of the Aromatic-responsive sigma 54-Dependent Regulator DmpR. J. Biol. Chem. 274: 32425-32432 [Abstract] [Full Text]  
• Arenghi, F. L. G., Pinti, M., Galli, E., Barbieri, P. (1999). Identification of the Pseudomonas stutzeri OX1 Toluene-o-Xylene Monooxygenase Regulatory Gene (touR) and of Its Cognate Promoter. Appl. Environ. Microbiol. 65: 4057-4063 [Abstract] [Full Text]  
• Willardson, B. M., Wilkins, J. F., Rand, T. A., Schupp, J. M., Hill, K. K., Keim, P., Jackson, P. J. (1998). Development and Testing of a Bacterial Biosensor for Toluene-Based Environmental Contaminants. Appl. Environ. Microbiol. 64: 1006-1012 [Abstract] [Full Text]  
• Ampe, F., Léonard, D., Lindley, N. D. (1998). Repression of Phenol Catabolism by Organic Acids in Ralstonia eutropha. Appl. Environ. Microbiol. 64: 1-6 [Abstract] [Full Text]  
• Ng, L., O'Neill, E., Shingler, V. (1996). Genetic Evidence for Interdomain Regulation of the Phenol-responsive &sfgr;54-dependent Activator DmpR. J. Biol. Chem. 271: 17281-17286 [Abstract] [Full Text]  
• Pérez-Martín, J., de Lorenzo, Víc. (1996). Identification of the Repressor Subdomain within the Signal Reception Module of the Prokaryotic Enhancer-binding Protein XylR of Pseudomonas putida. J. Biol. Chem. 271: 7899-7902 [Abstract] [Full Text]  
• Delgado, A.ón, Salto, R., Marqués, S., Ramos, J. L. (1995). Single Amino Acids Changes in the Signal Receptor Domain of XylR Resulted in Mutants That Stimulate Transcription in the Absence of Effectors. J. Biol. Chem. 270: 5144-5150 [Abstract] [Full Text]