This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kutter, E M Articles by Guttman, B S Search for Related Content PubMed PubMed Citation Articles by Kutter, E M Articles by Guttman, B S

 Previous Article  |  Next Article 

J Bacteriol. 1994 March; 176(6): 1647-1654

research-article

Identification of bacteriophage T4 prereplicative proteins on two-dimensional polyacrylamide gels.

Evergreen State College, Olympia, Washington 98505.

ABSTRACT

Bacteriophage T4 makes a large number of prereplicative proteins, which are involved in directing the transition from host to phage functions, in producing the new T4 DNA, and in regulating transcriptional shifts. We have used two-dimensional gel electrophoresis (nonequilibrium pH gradient electrophoresis gels in the first dimension and sodium dodecyl sulfate-polyacrylamide gradient slab gels in the second) to identify a number of new prereplicative proteins. The products of many known genes are identified because they are missing in mutants with amber mutations of those genes, as analyzed by us and/or by previous workers. Some have also been identified by running purified proteins as markers on gels with labeled extracts from infected cells. Other proteins that are otherwise unknown are characterized as missing in infections with phage carrying certain large deletions and, in some cases, are correlated with sequence data.

This article has been cited by other articles:

• Miller, E. S., Kutter, E., Mosig, G., Arisaka, F., Kunisawa, T., Ruger, W. (2003). Bacteriophage T4 Genome. Microbiol. Mol. Biol. Rev. 67: 86-156 [Abstract] [Full Text]