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J Bacteriol. 1994 March; 176(6): 1790-1792

research-article

Identification of the Methanococcus voltae S-layer structural gene.

Department of Microbiology, University of Illinois, Urbana 61802.

ABSTRACT

We have established that the gene which we had previously identified as encoding the Methanococcus voltae P-type ATPase is, in fact, the structural gene for the M. voltae S-layer protein. This conclusion is based on a comparison of the N-terminal sequence of S-layer protein prepared by two independent methods with that derived from the nucleotide sequence of the cloned gene. This conclusion was further supported by immunocytochemical localization of the antigen directed against the antibodies used in the cloning experiments.

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