This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Black, T A Articles by Wolk, C P Search for Related Content PubMed PubMed Citation Articles by Black, T A Articles by Wolk, C P

 Previous Article  |  Next Article 

J Bacteriol. 1994 April; 176(8): 2282-2292

research-article

Analysis of a Het- mutation in Anabaena sp. strain PCC 7120 implicates a secondary metabolite in the regulation of heterocyst spacing.

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing 48824.

ABSTRACT

Transposon-generated mutant N10 of Anabaena sp. strain PCC 7120 has a Het- phenotype (A. Ernst, T. Black, Y. Cai, J.-M. Panoff, D. N. Tiwari, and C. P. Wolk, J. Bacteriol. 174:6025-6032, 1992). Reconstruction of the transposon mutation reproduced a Het- phenotype, but reconstructions with other insertions at the position of the transposon produced strains that form multiple contiguous heterocysts. Sequence analysis around the site of insertion of the transposon showed that the insertion lies within the 5' end of an 861-bp open reading frame (ORF) (hetN). The product of translation of hetN (HetN) shows extensive similarity to NAD(P)H-dependent oxidoreductases that are involved in biosyntheses of fatty acids, poly-beta-hydroxybutyrate, nod factor, and polyketides. A second, 1,518-bp ORF (hetM) that ends 556 bp 5' from the start of hetN appears to encode a protein that has at least two functional domains: its amino terminus is similar to an acyl carrier protein, while its central portion is similar to domains of proteins that perform reductive reactions. A third, 711-bp ORF (hetI) encoded on the opposite strand ends 42 bp away from the 3' end of hetN. The protein encoded by hetI, HetI, is similar to Sfp from Bacillus subtilis and EntD from Escherichia coli, proteins that are required for the biosynthesis or export of cyclic peptides. Clones from a lambda-EMBL3 library that contain the wild-type DNA for hetN do not complement the hetN::Tn5-1063 mutation in N10. The presence of hetN, as the only ORF, on a replicating plasmid suppresses heterocyst formation in wild-type cells, whereas the additional presence of hetI alleviates this effect.

This article has been cited by other articles:

• Neunuebel, M. R., Golden, J. W. (2008). The Anabaena sp. Strain PCC 7120 Gene all2874 Encodes a Diguanylate Cyclase and Is Required for Normal Heterocyst Development under High-Light Growth Conditions. J. Bacteriol. 190: 6829-6836 [Abstract] [Full Text]  
• Chapman, K. E., Duggan, P. S., Billington, N. A. née, Adams, D. G. (2008). Mutation at Different Sites in the Nostoc punctiforme cyaC Gene, Encoding the Multiple-Domain Enzyme Adenylate Cyclase, Results in Different Levels of Infection of the Host Plant Blasia pusilla. J. Bacteriol. 190: 1843-1847 [Abstract] [Full Text]  
• Wang, Y., Lechno-Yossef, S., Gong, Y., Fan, Q., Wolk, C. P., Xu, X. (2007). Predicted Glycosyl Transferase Genes Located outside the HEP Island Are Required for Formation of Heterocyst Envelope Polysaccharide in Anabaena sp. Strain PCC 7120. J. Bacteriol. 189: 5372-5378 [Abstract] [Full Text]  
• Soule, T., Stout, V., Swingley, W. D., Meeks, J. C., Garcia-Pichel, F. (2007). Molecular Genetics and Genomic Analysis of Scytonemin Biosynthesis in Nostoc punctiforme ATCC 29133. J. Bacteriol. 189: 4465-4472 [Abstract] [Full Text]  
• Lechno-Yossef, S., Fan, Q., Ehira, S., Sato, N., Wolk, C. P. (2006). Mutations in Four Regulatory Genes Have Interrelated Effects on Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 188: 7387-7395 [Abstract] [Full Text]  
• Shi, Y., Zhao, W., Zhang, W., Ye, Z., Zhao, J. (2006). Regulation of intracellular free calcium concentration during heterocyst differentiation by HetR and NtcA in Anabaena sp. PCC 7120. Proc. Natl. Acad. Sci. USA 103: 11334-11339 [Abstract] [Full Text]  
• Johnson, M. A., Peti, W., Herrmann, T., Wilson, I. A., Wuthrich, K. (2006). Solution structure of Asl1650, an acyl carrier protein from Anabaena sp. PCC 7120 with a variant phosphopantetheinylation-site sequence. Protein Sci. 15: 1030-1041 [Abstract] [Full Text]  
• Copp, J. N., Neilan, B. A. (2006). The Phosphopantetheinyl Transferase Superfamily: Phylogenetic Analysis and Functional Implications in Cyanobacteria. Appl. Environ. Microbiol. 72: 2298-2305 [Abstract] [Full Text]  
• Orozco, C. C., Risser, D. D., Callahan, S. M. (2006). Epistasis Analysis of Four Genes from Anabaena sp. Strain PCC 7120 Suggests a Connection between PatA and PatS in Heterocyst Pattern Formation.. J. Bacteriol. 188: 1808-1816 [Abstract] [Full Text]  
• Sakr, S., Jeanjean, R., Zhang, C.-C., Arcondeguy, T. (2006). Inhibition of Cell Division Suppresses Heterocyst Development in Anabaena sp. Strain PCC 7120. J. Bacteriol. 188: 1396-1404 [Abstract] [Full Text]  
• Pratte, B. S., Thiel, T. (2006). High-Affinity Vanadate Transport System in the Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 188: 464-468 [Abstract] [Full Text]  
• Wang, Y., Xu, X. (2005). Regulation by hetC of Genes Required for Heterocyst Differentiation and Cell Division in Anabaena sp. Strain PCC 7120. J. Bacteriol. 187: 8489-8493 [Abstract] [Full Text]  
• Jenke-Kodama, H., Sandmann, A., Muller, R., Dittmann, E. (2005). Evolutionary Implications of Bacterial Polyketide Synthases. Mol Biol Evol 22: 2027-2039 [Abstract] [Full Text]  
• Zhao, Y., Shi, Y., Zhao, W., Huang, X., Wang, D., Brown, N., Brand, J., Zhao, J. (2005). From the Cover: CcbP, a calcium-binding protein from Anabaena sp. PCC 7120, provides evidence that calcium ions regulate heterocyst differentiation. Proc. Natl. Acad. Sci. USA 102: 5744-5748 [Abstract] [Full Text]  
• Ramirez, M. E., Hebbar, P. B., Zhou, R., Wolk, C. P., Curtis, S. E. (2005). Anabaena sp. Strain PCC 7120 Gene devH Is Required for Synthesis of the Heterocyst Glycolipid Layer. J. Bacteriol. 187: 2326-2331 [Abstract] [Full Text]  
• Khudyakov, I. Y., Golden, J. W. (2004). Different functions of HetR, a master regulator of heterocyst differentiation in Anabaena sp. PCC 7120, can be separated by mutation. Proc. Natl. Acad. Sci. USA 101: 16040-16045 [Abstract] [Full Text]  
• Wu, X., Liu, D., Lee, M. H., Golden, J. W. (2004). patS Minigenes Inhibit Heterocyst Development of Anabaena sp. Strain PCC 7120. J. Bacteriol. 186: 6422-6429 [Abstract] [Full Text]  
• Huang, X., Dong, Y., Zhao, J. (2004). HetR homodimer is a DNA-binding protein required for heterocyst differentiation, and the DNA-binding activity is inhibited by PatS. Proc. Natl. Acad. Sci. USA 101: 4848-4853 [Abstract] [Full Text]  
• Ning, D., Xu, X. (2004). alr0117, a two-component histidine kinase gene, is involved in heterocyst development in Anabaena sp. PCC 7120. Microbiology 150: 447-453 [Abstract] [Full Text]  
• Liu, D., Golden, J. W. (2002). hetL Overexpression Stimulates Heterocyst Formation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 184: 6873-6881 [Abstract] [Full Text]  
• Meeks, J. C., Elhai, J. (2002). Regulation of Cellular Differentiation in Filamentous Cyanobacteria in Free-Living and Plant-Associated Symbiotic Growth States. Microbiol. Mol. Biol. Rev. 66: 94-121 [Abstract] [Full Text]  
• Zhu, J., Jager, K., Black, T., Zarka, K., Koksharova, O., Wolk, C. P. (2001). HcwA, an Autolysin, Is Required for Heterocyst Maturation in Anabaena sp. Strain PCC 7120. J. Bacteriol. 183: 6841-6851 [Abstract] [Full Text]  
• Gaitatzis, N., Kunze, B., Muller, R. (2001). In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases. Proc. Natl. Acad. Sci. USA 10.1073/pnas.201167098v1 [Abstract] [Full Text]  
• Wang, L., McVey, J., Vining, L. C. (2001). Cloning and functional analysis of a phosphopantetheinyl transferase superfamily gene associated with jadomycin biosynthesis in Streptomyces venezuelae ISP5230. Microbiology 147: 1535-1545 [Abstract] [Full Text]  
• Güler, S., Essigmann, B., Benning, C. (2000). A Cyanobacterial Gene, sqdX, Required for Biosynthesis of the Sulfolipid Sulfoquinovosyldiacylglycerol. J. Bacteriol. 182: 543-545 [Abstract] [Full Text]  
• Schwartz, S. H., Black, T. A., Jäger, K., Panoff, J.-M., Wolk, C. P. (1998). Regulation of an Osmoticum-Responsive Gene in Anabaena sp. Strain PCC 7120. J. Bacteriol. 180: 6332-6337 [Abstract] [Full Text]  
• Zhu, J., Kong, R., Wolk, C. P. (1998). Regulation of hepA of Anabaena sp. Strain PCC 7120 by Elements 5' from the Gene and by hepK. J. Bacteriol. 180: 4233-4242 [Abstract] [Full Text]  
• Zhang, C.-C., Friry, A., Peng, L. (1998). Molecular and Genetic Analysis of Two Closely Linked Genes That Encode, Respectively, a Protein Phosphatase 1/2A/2B Homolog and a Protein Kinase Homolog in the Cyanobacterium Anabaena sp. Strain PCC 7120. J. Bacteriol. 180: 2616-2622 [Abstract] [Full Text]  
• Froehlich, J. E., Benning, C., Dormann, P. (2001). The Digalactosyldiacylglycerol (DGDG) Synthase DGD1 Is Inserted into the Outer Envelope Membrane of Chloroplasts in a Manner Independent of the General Import Pathway and Does Not Depend on Direct Interaction with Monogalactosyldiacylglycerol Synthase for DGDG Biosynthesis. J. Biol. Chem. 276: 31806-31812 [Abstract] [Full Text]  
• Gaitatzis, N., Kunze, B., Muller, R. (2001). In vitro reconstitution of the myxochelin biosynthetic machinery of Stigmatella aurantiaca Sg a15: Biochemical characterization of a reductive release mechanism from nonribosomal peptide synthetases. Proc. Natl. Acad. Sci. USA 98: 11136-11141 [Abstract] [Full Text]