This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhao, N Articles by Larson, T J Search for Related Content PubMed PubMed Citation Articles by Zhao, N Articles by Larson, T J

 Previous Article  |  Next Article 

J Bacteriol. 1994 April; 176(8): 2393-2397

research-article

Characterization of the interaction of the glp repressor of Escherichia coli K-12 with single and tandem glp operator variants.

Department of Biochemistry and Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061-0308.

ABSTRACT

The glp operons of Escherichia coli are negatively controlled by the glp repressor. Comparison of the repressor-binding affinities for consensus and altered consensus operators in vivo showed that all base substitutions at positions 3, 4, 5, and 8 from the center of the palindromic operator caused a striking decrease in repressor binding. Substitutions at other positions had a severe to no effect on repressor binding, depending on the base substitution. The results obtained indicate that the repressor binds with highest affinity to operators with the half-site WATKYTCGWW, where W is A or T, K is G or T, and Y is C or T. Strong cooperative binding of the repressor to tandem operators was demonstrated in vivo. Cooperativity was maximal when two 20-bp operators were directly repeated or when 2 bp separated the two operators. Cooperativity decreased with the deletion of 2 bp or the addition of 4 bp between the individual operators. Cooperativity was eliminated with a 6-bp insertion between the operators.

This article has been cited by other articles:

• Song, X.-M., Janson, H. (2003). Differences in Genetic and Transcriptional Organization of the glpTQ Operons between Haemophilus influenzae Type b and Nontypeable Strains. J. Bacteriol. 185: 7285-7290 [Abstract] [Full Text]  
• Eppler, T., Postma, P., Schutz, A., Volker, U., Boos, W. (2002). Glycerol-3-Phosphate-Induced Catabolite Repression in Escherichia coli. J. Bacteriol. 184: 3044-3052 [Abstract] [Full Text]  
• Holtman, C. K., Pawlyk, A. C., Meadow, N. D., Pettigrew, D. W. (2001). Reverse Genetics of Escherichia coli Glycerol Kinase Allosteric Regulation and Glucose Control of Glycerol Utilization In Vivo. J. Bacteriol. 183: 3336-3344 [Abstract] [Full Text]  
• Gerischer, U., Segura, A., Ornston, L. N. (1998). PcaU, a Transcriptional Activator of Genes for Protocatechuate Utilization in Acinetobacter. J. Bacteriol. 180: 1512-1524 [Abstract] [Full Text]