This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kwakman, J H Articles by Postma, P W Search for Related Content PubMed PubMed Citation Articles by Kwakman, J H Articles by Postma, P W

 Previous Article  |  Next Article 

J Bacteriol. 1994 May; 176(9): 2694-2698

Glucose kinase has a regulatory role in carbon catabolite repression in Streptomyces coelicolor.

E.C. Slater Institute, BioCentrum Amsterdam, University of Amsterdam, The Netherlands.

ABSTRACT

A glucose kinase (glkA) mutant of Streptomyces coelicolor A3(2) M145 was selected by the ability to grow in the presence of the nonmetabolizable glucose analog 2-deoxyglucose. In this glkA mutant, carbon catabolite repression of glycerol kinase and agarase was relieved on several carbon sources tested, even though most of these carbon sources are not metabolized via glucose kinase. This suggests that catabolite repression is not regulated by the flux through glucose kinase and that the protein itself has a regulatory role in carbon catabolite repression. A 10-fold overproduction of glucose kinase also results in relief of catabolite repression, suggesting that excess glucose kinase can titrate the repressing signal away. This could be achieved directly by competition of excess glucose kinase with its repressing form for binding sites on DNA promoter regions or indirectly by competition for binding of another regulatory protein.

This article has been cited by other articles:

• Lu, G.-T., Yang, Z.-J., Peng, F.-Y., Tan, Y.-N., Tang, Y.-Q., Feng, J.-X., Tang, D.-J., He, Y.-Q., Tang, J.-L. (2007). The role of glucose kinase in carbohydrate utilization and extracellular polysaccharide production in Xanthomonas campestris pathovar campestris. Microbiology 153: 4284-4294 [Abstract] [Full Text]  
• Saulnier, D. M. A., Molenaar, D., de Vos, W. M., Gibson, G. R., Kolida, S. (2007). Identification of Prebiotic Fructooligosaccharide Metabolism in Lactobacillus plantarum WCFS1 through Microarrays. Appl. Environ. Microbiol. 73: 1753-1765 [Abstract] [Full Text]  
• Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
• Hillerich, B., Westpheling, J. (2006). A New GntR Family Transcriptional Regulator in Streptomyces coelicolor Is Required for Morphogenesis and Antibiotic Production and Controls Transcription of an ABC Transporter in Response to Carbon Source. J. Bacteriol. 188: 7477-7487 [Abstract] [Full Text]  
• Ryan, S. M., Fitzgerald, G. F., van Sinderen, D. (2005). Transcriptional Regulation and Characterization of a Novel {beta}-Fructofuranosidase-Encoding Gene from Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 71: 3475-3482 [Abstract] [Full Text]  
• Caescu, C. I., Vidal, O., Krzewinski, F., Artenie, V., Bouquelet, S. (2004). Bifidobacterium longum Requires a Fructokinase (Frk; ATP:D-Fructose 6-Phosphotransferase, EC 2.7.1.4) for Fructose Catabolism. J. Bacteriol. 186: 6515-6525 [Abstract] [Full Text]  
• Bertram, R., Schlicht, M., Mahr, K., Nothaft, H., Saier, M. H. Jr., Titgemeyer, F. (2004). In Silico and Transcriptional Analysis of Carbohydrate Uptake Systems of Streptomyces coelicolor A3(2). J. Bacteriol. 186: 1362-1373 [Abstract] [Full Text]  
• Nothaft, H., Parche, S., Kamionka, A., Titgemeyer, F. (2003). In Vivo Analysis of HPr Reveals a Fructose-Specific Phosphotransferase System That Confers High-Affinity Uptake in Streptomyces coelicolor. J. Bacteriol. 185: 929-937 [Abstract] [Full Text]  
• Fields, M. W., Russell, J. B. (2001). The glucomannokinase of Prevotella bryantii B14 and its potential role in regulating {beta}-glucanase expression. Microbiology 147: 1035-1043 [Abstract] [Full Text]  
• Huynh, P. L., Jankovic, I., Schnell, N. F., Brückner, R. (2000). Characterization of an HPr Kinase Mutant of Staphylococcus xylosus. J. Bacteriol. 182: 1895-1902 [Abstract] [Full Text]  
• Saito, A., Fujii, T., Yoneyama, T., Miyashita, K. (1998). glkA Is Involved in Glucose Repression of Chitinase Production in Streptomyces lividans. J. Bacteriol. 180: 2911-2914 [Abstract] [Full Text]  
• Pope, M. K., Green, B., Westpheling, J. (1998). The bldB Gene Encodes a Small Protein Required for Morphogenesis, Antibiotic Production, and Catabolite Control in Streptomyces coelicolor. J. Bacteriol. 180: 1556-1562 [Abstract] [Full Text]  
• Ni, X., Westpheling, J. (1997). Direct repeat sequences in the Streptomyces chitinase-63 promoter direct both glucose repression and chitin induction. Proc. Natl. Acad. Sci. USA 94: 13116-13121 [Abstract] [Full Text]