This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tao, T. Articles by Maguire, M. E. Search for Related Content PubMed PubMed Citation Articles by Tao, T. Articles by Maguire, M. E.

 Previous Article  |  Next Article 

J. Bacteriol., May 1995, 2654-2662, Vol 177, No. 10
Copyright © 1995, American Society for Microbiology

Magnesium transport in Salmonella typhimurium: mgtA encodes a P-type ATPase and is regulated by Mg2+ in a manner similar to that of the mgtB P-type ATPase

T Tao, MD Snavely, SG Farr and ME Maguire
Department of Pharmacology, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106-4965, USA.

Salmonella typhimurium has three distinct Mg2+ transport systems: CorA, MgtA, and MgtB, each encoded by its respective gene. corA and mgtB have been previously sequenced and characterized. This report details the sequence and properties of mgtA. Like mgtB, mgtA encodes a P-type ATPase. The mgtA gene encodes a slightly smaller protein than does mgtB, with a predicted molecular mass of about 95 kDa, running at 91 kDa on protein gels, which compares with values of 101 and 102 kDa, respectively, for the MgtB protein. The deduced amino acid sequence of MgtA is only 50% identical to that of MgtB, with a further 25% conservative amino acid substitutions, surprisingly low for such otherwise functionally similar proteins from the same organism. Codon usage for each gene is normal for S. typhimurium, however, indicating that neither gene is the result of a recent acquisition from another organism. A single open reading frame at mgtA encodes MgtA, in contrast to mgtB, which is shown to be an operon encoding (5' to 3') the 22.5- kDa MgtC and the MgtB proteins. Genetic constructs were used to show that deletion of MgtC does not alter the expression or transport properties of MgtB, making the role of the companion MgtC protein unclear. (The S. typhimurium homolog of treR, which encodes a putative repressor for trehalose uptake, is encoded by a gene adjacent to mgtA, and its sequence is also reported.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

• Papp-Wallace, K. M., Maguire, M. E. (2008). Regulation of CorA Mg2+ Channel Function Affects the Virulence of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 190: 6509-6516 [Abstract] [Full Text]  
• Papp-Wallace, K. M., Nartea, M., Kehres, D. G., Porwollik, S., McClelland, M., Libby, S. J., Fang, F. C., Maguire, M. E. (2008). The CorA Mg2+ Channel Is Required for the Virulence of Salmonella enterica Serovar Typhimurium. J. Bacteriol. 190: 6517-6523 [Abstract] [Full Text]  
• Wang, S.-Z., Chen, Y., Sun, Z.-H., Zhou, Q., Sui, S.-F. (2006). Escherichia coli CorA Periplasmic Domain Functions as a Homotetramer to Bind Substrate. J. Biol. Chem. 281: 26813-26820 [Abstract] [Full Text]  
• Gunzel, D., Kucharski, L. M., Kehres, D. G., Romero, M. F., Maguire, M. E. (2006). The MgtC Virulence Factor of Salmonella enterica Serovar Typhimurium Activates Na+,K+-ATPase.. J. Bacteriol. 188: 5586-5594 [Abstract] [Full Text]  
• McPhee, J. B., Bains, M., Winsor, G., Lewenza, S., Kwasnicka, A., Brazas, M. D., Brinkman, F. S. L., Hancock, R. E. W. (2006). Contribution of the PhoP-PhoQ and PmrA-PmrB Two-Component Regulatory Systems to Mg2+-Induced Gene Regulation in Pseudomonas aeruginosa. J. Bacteriol. 188: 3995-4006 [Abstract] [Full Text]  
• Lavigne, J.-P., O'Callaghan, D., Blanc-Potard, A.-B. (2005). Requirement of MgtC for Brucella suis Intramacrophage Growth: a Potential Mechanism Shared by Salmonella enterica and Mycobacterium tuberculosis for Adaptation to a Low-Mg2+ Environment. Infect. Immun. 73: 3160-3163 [Abstract] [Full Text]  
• Papp, K. M., Maguire, M. E. (2004). The CorA Mg2+ Transporter Does Not Transport Fe2+. J. Bacteriol. 186: 7653-7658 [Abstract] [Full Text]  
• Lejona, S., Aguirre, A., Cabeza, M. L., Vescovi, E. G., Soncini, F. C. (2003). Molecular Characterization of the Mg2+-Responsive PhoP-PhoQ Regulon in Salmonella enterica. J. Bacteriol. 185: 6287-6294 [Abstract] [Full Text]  
• Watt, R. K., Ludden, P. W. (1999). Ni2+ Transport and Accumulation in Rhodospirillum rubrum. J. Bacteriol. 181: 4554-4560 [Abstract] [Full Text]  
• Kakinuma, Y. (1998). Inorganic Cation Transport and Energy Transduction in Enterococcus hirae and Other Streptococci. Microbiol. Mol. Biol. Rev. 62: 1021-1045 [Abstract] [Full Text]  
• Smith, R. L., Szegedy, M. A., Kucharski, L. M., Walker, C., Wiet, R. M., Redpath, A., Kaczmarek, M. T., Maguire, M. E. (1998). The CorA Mg2+ Transport Protein of Salmonella typhimurium. MUTAGENESIS OF CONSERVED RESIDUES IN THE THIRD MEMBRANE DOMAIN IDENTIFIES A Mg2+ PORE. J. Biol. Chem. 273: 28663-28669 [Abstract] [Full Text]  
• Moncrief, M. B. C., Maguire, M. E. (1998). Magnesium and the Role of mgtC in Growth of Salmonella typhimurium. Infect. Immun. 66: 3802-3809 [Abstract] [Full Text]  
• Smith, R. L., Gottlieb, E., Kucharski, L. M., Maguire, M. E. (1998). Functional Similarity between Archaeal and Bacterial CorA Magnesium Transporters. J. Bacteriol. 180: 2788-2791 [Abstract] [Full Text]  
• Horlacher, R., Boos, W. (1997). Characterization of TreR, the Major Regulator of the Escherichia coli Trehalose System. J. Biol. Chem. 272: 13026-13032 [Abstract] [Full Text]