This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bidnenko, E. Articles by Chopin, M. C. Search for Related Content PubMed PubMed Citation Articles by Bidnenko, E. Articles by Chopin, M. C.

 Previous Article  |  Next Article 

J. Bacteriol., 07 1995, 3824-3829, Vol 177, No. 13
Copyright © 1995, American Society for Microbiology

Phage operon involved in sensitivity to the Lactococcus lactis abortive infection mechanism AbiD1

E Bidnenko, D Ehrlich and MC Chopin
Laboratoire de Genetique Microbienne, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.

Phage bIL66 is unable to grow on Lactococcus lactis cells harboring the abortive infection gene abiD1. Spontaneous phage mutants able to grow on AbiD1 cells were used to study phage-Abi interaction. A 1.33-kb DNA segment of a mutant phage allowed growth of AbiD1s phages in AbiD1 cells when present in trans. Sequence analysis of this segment revealed an operon composed of four open reading frames, designated orf1 to orf4. The operon is transcribed 10 min after infection from a promoter presenting an extended -10 consensus sequence but no -35 sequence. Analysis of four independent AbiD1r mutants revealed a different point mutation localized in orf1, implying that this open reading frame is needed for sensitivity to AbiD1. However, the sensitivity is partly suppressed when orf3 is expressed in trans on a high-copy-number plasmid, suggesting that AbiD1 acts by decreasing the concentration of an available orf3 product.

This article has been cited by other articles:

• Yang, J. M., DeUrraza, P. J., Matvienko, N., O'Sullivan, D. J. (2006). Involvement of the LlaKR2I Methylase in Expression of the AbiR Bacteriophage Defense System in Lactococcus lactis subsp. lactis biovar diacetylactis KR2.. J. Bacteriol. 188: 1920-1928 [Abstract] [Full Text]  
• Madera, C., Monjardin, C., Suarez, J. E. (2004). Milk Contamination and Resistance to Processing Conditions Determine the Fate of Lactococcus lactis Bacteriophages in Dairies. Appl. Environ. Microbiol. 70: 7365-7371 [Abstract] [Full Text]  
• Dupont, K., Vogensen, F. K., Neve, H., Bresciani, J., Josephsen, J. (2004). Identification of the Receptor-Binding Protein in 936-Species Lactococcal Bacteriophages. Appl. Environ. Microbiol. 70: 5818-5824 [Abstract] [Full Text]  
• Dupont, K., Janzen, T., Vogensen, F. K., Josephsen, J., Stuer-Lauridsen, B. (2004). Identification of Lactococcus lactis Genes Required for Bacteriophage Adsorption. Appl. Environ. Microbiol. 70: 5825-5832 [Abstract] [Full Text]  
• Domingues, S., Chopin, A., Ehrlich, S. D., Chopin, M.-C. (2004). A Phage Protein Confers Resistance to the Lactococcal Abortive Infection Mechanism AbiP. J. Bacteriol. 186: 3278-3281 [Abstract] [Full Text]  
• Domingues, S., Chopin, A., Ehrlich, S. D., Chopin, M.-C. (2004). The Lactococcal Abortive Phage Infection System AbiP Prevents both Phage DNA Replication and Temporal Transcription Switch. J. Bacteriol. 186: 713-721 [Abstract] [Full Text]  
• Jankovic, I., Ventura, M., Meylan, V., Rouvet, M., Elli, M., Zink, R. (2003). Contribution of Aggregation-Promoting Factor to Maintenance of Cell Shape in Lactobacillusgasseri 4B2. J. Bacteriol. 185: 3288-3296 [Abstract] [Full Text]  
• Bouchard, J. D., Dion, E., Bissonnette, F., Moineau, S. (2002). Characterization of the Two-Component Abortive Phage Infection Mechanism AbiT from Lactococcus lactis. J. Bacteriol. 184: 6325-6332 [Abstract] [Full Text]  
• Crutz-Le Coq, A.-M., Cesselin, B., Commissaire, J., Anba, J. (2002). Sequence analysis of the lactococcal bacteriophage bIL170: insights into structural proteins and HNH endonucleases in dairy phages. Microbiology 148: 985-1001 [Abstract] [Full Text]  
• Seegers, J. F. M. L., van Sinderen, D., Fitzgerald, G. F. (2000). Molecular characterization of the lactococcal plasmid pCIS3: natural stacking of specificity subunits of a type I restriction/modification system in a single lactococcal strain. Microbiology 146: 435-443 [Abstract] [Full Text]  
• Boucher, I., Émond, E., Dion, E., Montpetit, D., Moineau, S. (2000). Microbiological and molecular impacts of AbiK on the lytic cycle of Lactococcus lactis phages of the 936 and P335 species. Microbiology 146: 445-453 [Abstract] [Full Text]  
• Emond, E., Dion, E., Walker, S. A., Vedamuthu, E. R., Kondo, J. K., Moineau, S. (1998). AbiQ, an Abortive Infection Mechanism from Lactococcus lactis. Appl. Environ. Microbiol. 64: 4748-4756 [Abstract] [Full Text]  
• Bohn, C., Bouloc, P. (1998). The Escherichia coli cmlA Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-beta -D-Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity. J. Bacteriol. 180: 6072-6075 [Abstract] [Full Text]  
• Lubbers, M. W., Schofield, K., Waterfield, N. R., Polzin, K. M. (1998). Transcription Analysis of the Prolate-Headed Lactococcal Bacteriophage c2. J. Bacteriol. 180: 4487-4496 [Abstract] [Full Text]