This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kasberg, T. Articles by Reineke, W. Search for Related Content PubMed PubMed Citation Articles by Kasberg, T. Articles by Reineke, W.

 Previous Article  |  Next Article 

J. Bacteriol., Jul 1995, 3885-3889, Vol 177, No. 13
Copyright © 1995, American Society for Microbiology

Evidence that operons tcb, tfd, and clc encode maleylacetate reductase, the fourth enzyme of the modified ortho pathway

T Kasberg, DL Daubaras, AM Chakrabarty, D Kinzelt and W Reineke
Bergische Universitat-Gesamthochschule Wuppertal, Germany.

The maleylacetate reductase from Pseudomonas sp. strain B13 functioning in the modified ortho pathway was purified and digested with trypsin. The polypeptides separated by high-performance liquid chromatography were sequenced. Alignments with the polypeptides predicted from the tfdF and tcbF genes located on plasmids pJP4 of the 2,4- dichlorophenoxyacetate-degrading Alcaligenes eutrophus JMP134 and pP51 of the 1,2,4-trichlorobenzene-degrading Pseudomonas sp. strain P51 as well as polypeptides predicted from the tftE gene located on the chromosome of the 2,4,5-trichlorophenoxyacetate-degrading Burkholderia cepacia AC1100 were obtained. In addition, the deduced protein sequence encoded by the nucleotide sequence downstream of clcD on plasmid pAC27 of the 3-chlorobenzoate-degrading Pseudomonas putida AC866 was tested for homology. Significant sequence similarities with the polypeptides encoded by the tfdF, tcbF, and tftE genes as well as the nucleotide sequence downstream of the clcD gene gave evidence that these genes might encode maleylacetate reductases. A NAD-binding motif in a beta alpha beta-element was detected.

This article has been cited by other articles:

• Gaillard, M., Vallaeys, T., Vorholter, F. J., Minoia, M., Werlen, C., Sentchilo, V., Puhler, A., van der Meer, J. R. (2006). The clc Element of Pseudomonas sp. Strain B13, a Genomic Island with Various Catabolic Properties.. J. Bacteriol. 188: 1999-2013 [Abstract] [Full Text]  
• Endo, R., Kamakura, M., Miyauchi, K., Fukuda, M., Ohtsubo, Y., Tsuda, M., Nagata, Y. (2005). Identification and Characterization of Genes Involved in the Downstream Degradation Pathway of {gamma}-Hexachlorocyclohexane in Sphingomonas paucimobilis UT26. J. Bacteriol. 187: 847-853 [Abstract] [Full Text]  
• Seibert, V., Thiel, M., Hinner, I.-S., Schlomann, M. (2004). Characterization of a gene cluster encoding the maleylacetate reductase from Ralstonia eutropha 335T, an enzyme recruited for growth with 4-fluorobenzoate. Microbiology 150: 463-472 [Abstract] [Full Text]  
• Ledger, T., Pieper, D. H., Perez-Pantoja, D., Gonzalez, B. (2002). Novel insights into the interplay between peripheral reactions encoded by xyl genes and the chlorocatechol pathway encoded by tfd genes for the degradation of chlorobenzoates by Ralstonia eutropha JMP134. Microbiology 148: 3431-3440 [Abstract] [Full Text]  
• Plumeier, I., Perez-Pantoja, D., Heim, S., Gonzalez, B., Pieper, D. H. (2002). Importance of Different tfd Genes for Degradation of Chloroaromatics by Ralstonia eutropha JMP134. J. Bacteriol. 184: 4054-4064 [Abstract] [Full Text]  
• Pieper, D. H., Pollmann, K., Nikodem, P., Gonzalez, B., Wray, V. (2002). Monitoring Key Reactions in Degradation of Chloroaromatics by In Situ 1H Nuclear Magnetic Resonance: Solution Structures of Metabolites Formed from cis-Dienelactone. J. Bacteriol. 184: 1466-1470 [Abstract] [Full Text]  
• Roane, T. M., Josephson, K. L., Pepper, I. L. (2001). Dual-Bioaugmentation Strategy To Enhance Remediation of Cocontaminated Soil. Appl. Environ. Microbiol. 67: 3208-3215 [Abstract] [Full Text]  
• Pérez-Pantoja, D., Guzmán, L., Manzano, M., Pieper, D. H., González, B. (2000). Role of tfdCIDIEIFI and tfdDIICIIEIIFII Gene Modules in Catabolism of 3-Chlorobenzoate by Ralstonia eutropha JMP134(pJP4). Appl. Environ. Microbiol. 66: 1602-1608 [Abstract] [Full Text]  
• Miyauchi, K., Adachi, Y., Nagata, Y., Takagi, M. (1999). Cloning and Sequencing of a Novel meta-Cleavage Dioxygenase Gene Whose Product Is Involved in Degradation of gamma -Hexachlorocyclohexane in Sphingomonas paucimobilis. J. Bacteriol. 181: 6712-6719 [Abstract] [Full Text]  
• Ogawa, N., Miyashita, K. (1999). The Chlorocatechol-Catabolic Transposon Tn5707 of Alcaligenes eutrophus NH9, Carrying a Gene Cluster Highly Homologous to That in the 1,2,4-Trichlorobenzene-Degrading Bacterium Pseudomonas sp. Strain P51, Confers the Ability To Grow on 3-Chlorobenzoate. Appl. Environ. Microbiol. 65: 724-731 [Abstract] [Full Text]  
• Seibert, V., Kourbatova, E. M., Golovleva, L. A., Schlömann, M. (1998). Characterization of the Maleylacetate Reductase MacA of Rhodococcus opacus 1CP and Evidence for the Presence of an Isofunctional Enzyme. J. Bacteriol. 180: 3503-3508 [Abstract] [Full Text]