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J. Bacteriol., Aug 1995, 4230-4237, Vol 177, No. 15
A Allaoui, R Scheen, C Lambert de Rouvroit and GR Cornelis
Pathogenic yersiniae require Ca2+ for growth at 37 degrees C. They harbor
closely related plasmids of about 70 kb that are essential for virulence.
At 37 degrees C and in the absence of Ca2+ ions, these plasmids cause a
decrease in growth rate and the release of large amounts of proteins called
Yops. Here we describe the virG gene of Yersinia enterocolitica; virG is
located just upstream of the virF gene, which encodes the transcriptional
activator of some plasmid virulence factors. Analysis of the VirG amino
acid sequence suggested that virG encodes a lipoprotein, which was
confirmed by [3H]palmitate labeling of VirG-PhoA fusion proteins. A
nonpolar virG mutant was constructed and found to be Ca2+ independent for
growth at 37 degrees C but to still secrete Yops. This phenotype was
complemented by the introduction of a plasmid harboring an intact virG
gene. VirG was found to be homologous to ExsB, a protein encoded by a
Pseudomonas aeruginosa gene located in the locus controlling exoenzyme S
synthesis. Interestingly, the exsA gene, located just downstream of exsB,
is also homologous to virF.
Copyright © 1995, American Society for Microbiology
VirG, a Yersinia enterocolitica lipoprotein involved in Ca2+ dependency, is related to exsB of Pseudomonas aeruginosa
Microbial Pathogenesis Unit, Universite Catholique de Louvain, Brussels, Belgium.
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