This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Rosche, W. A. Articles by Sinden, R. R. Search for Related Content PubMed PubMed Citation Articles by Rosche, W. A. Articles by Sinden, R. R.

 Previous Article  |  Next Article 

J. Bacteriol., 08 1995, 4385-4391, Vol 177, No. 15
Copyright © 1995, American Society for Microbiology

Differential DNA secondary structure-mediated deletion mutation in the leading and lagging strands

WA Rosche, TQ Trinh and RR Sinden
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, Ohio 45267-0524, USA.

The frequencies of deletion of short sequences (mutation inserts) inserted into the chloramphenicol acetyl-transferase (CAT) gene were measured for pBR325 and pBR523, in which the orientation of the CAT gene was reversed, in Escherichia coli. Reversal of the CAT gene changes the relationship between the transcribed strand and the leading and lagging strands of the DNA replication fork in pBR325-based plasmids. Deletion of these mutation inserts may be mediated by slipped misalignment during DNA replication. Symmetrical sequences, in which the same potential DNA structural misalignment can form in both the leading and lagging strands, exhibited an approximately twofold difference in the deletion frequencies upon reversal of the CAT gene. Sequences that contained an inverted repeat that was asymmetric with respect to flanking direct repeats were designed. With asymmetric mutation inserts, different misaligned structural intermediates could form in the leading and lagging strands, depending on the orientation of the insert and/or of the CAT gene. When slippage could be stabilized by a hairpin in the lagging strand, thereby forming a three-way junction, deletion occurred by up to 50-fold more frequently than when this structure formed in the leading strand. These results support the model that slipped misalignment involving DNA secondary structure occurs preferentially in the lagging strand during DNA replication.

This article has been cited by other articles:

• Zahra, R., Blackwood, J. K., Sales, J., Leach, D. R. F. (2007). Proofreading and Secondary Structure Processing Determine the Orientation Dependence of CAG{middle dot}CTG Trinucleotide Repeat Instability in Escherichia coli. Genetics 176: 27-41 [Abstract] [Full Text]  
• Kogo, H., Inagaki, H., Ohye, T., Kato, T., Emanuel, B. S., Kurahashi, H. (2007). Cruciform extrusion propensity of human translocation-mediating palindromic AT-rich repeats. Nucleic Acids Res 35: 1198-1208 [Abstract] [Full Text]  
• Gray, S. J., Gerhardt, J., Doerfler, W., Small, L. E., Fanning, E. (2007). An Origin of DNA Replication in the Promoter Region of the Human Fragile X Mental Retardation (FMR1) Gene. Mol. Cell. Biol. 27: 426-437 [Abstract] [Full Text]  
• Kim, S.-H., Pytlos, M. J., Rosche, W. A., Sinden, R. R. (2006). (CAG){middle dot}(CTG) Repeats Associated with Neurodegenerative Diseases Are Stable in the Escherichia coli Chromosome. J. Biol. Chem. 281: 27950-27955 [Abstract] [Full Text]  
• Bzymek, M., Lovett, S. T. (2001). Instability of repetitive DNA sequences: The role of replication in multiple mechanisms. Proc. Natl. Acad. Sci. USA 98: 8319-8325 [Abstract] [Full Text]  
• Maliszewska-Tkaczyk, M., Jonczyk, P., Bialoskorska, M., Schaaper, R. M., Fijalkowska, I. J. (2000). SOS mutator activity: Unequal mutagenesis on leading and lagging strands. Proc. Natl. Acad. Sci. USA 10.1073/pnas.220424697v1 [Abstract] [Full Text]  
• Pan, X., Leach, D. R. F. (2000). The roles of mutS, sbcCD and recA in the propagation of TGG repeats in Escherichia coli. Nucleic Acids Res 28: 3178-3184 [Abstract] [Full Text]  
• Nasar, F., Jankowski, C., Nag, D. K. (2000). Long Palindromic Sequences Induce Double-Strand Breaks during Meiosis in Yeast. Mol. Cell. Biol. 20: 3449-3458 [Abstract] [Full Text]  
• Pilar Francino*, M., Ochman, H. (2000). Strand Symmetry Around the {beta}-Globin Origin of Replication in Primates. Mol Biol Evol 17: 416-422 [Abstract] [Full Text]  
• Cromie, G. A., Millar, C. B., Schmidt, K. H., Leach, D. R. F. (2000). Palindromes as Substrates for Multiple Pathways of Recombination in Escherichia coli. Genetics 154: 513-522 [Abstract] [Full Text]  
• Canceill, D., Viguera, E., Ehrlich, S. D. (1999). Replication Slippage of Different DNA Polymerases Is Inversely Related to Their Strand Displacement Efficiency. J. Biol. Chem. 274: 27481-27490 [Abstract] [Full Text]  
• Fijalkowska, I. J., Jonczyk, P., Tkaczyk, M. M., Bialoskorska, M., Schaaper, R. M. (1998). Unequal fidelity of leading strand and lagging strand DNA replication on the Escherichia coli chromosome. Proc. Natl. Acad. Sci. USA 95: 10020-10025 [Abstract] [Full Text]  
• van Belkum, A., Scherer, S., van Alphen, L., Verbrugh, H. (1998). Short-Sequence DNA Repeats in Prokaryotic Genomes. Microbiol. Mol. Biol. Rev. 62: 275-293 [Abstract] [Full Text]  
• Lobachev, K. S., Shor, B. M., Tran, H. T., Taylor, W., Keen, J. D., Resnick, M. A., Gordenin, D. A. (1998). Factors Affecting Inverted Repeat Stimulation of Recombination and Deletion in Saccharomyces cerevisiae. Genetics 148: 1507-1524 [Abstract] [Full Text]  
• Bacolla, A., Jaworski, A., Connors, T. D., Wells, R. D. (2001). PKD1 Unusual DNA Conformations Are Recognized by Nucleotide Excision Repair. J. Biol. Chem. 276: 18597-18604 [Abstract] [Full Text]  
• Maliszewska-Tkaczyk, M., Jonczyk, P., Bialoskorska, M., Schaaper, R. M., Fijalkowska, I. J. (2000). SOS mutator activity: Unequal mutagenesis on leading and lagging strands. Proc. Natl. Acad. Sci. USA 97: 12678-12683 [Abstract] [Full Text]