This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Endo, G. Articles by Silver, S. Search for Related Content PubMed PubMed Citation Articles by Endo, G. Articles by Silver, S.

 Previous Article  |  Next Article 

J. Bacteriol., Aug 1995, 4437-4441, Vol 177, No. 15
Copyright © 1995, American Society for Microbiology

CadC, the transcriptional regulatory protein of the cadmium resistance system of Staphylococcus aureus plasmid pI258

G Endo and S Silver
Department of Microbiology & Immunology, University of Illinois College of Medicine, Chicago 60612-7344, USA.

The CadC protein from the cadA cadmium resistance operon of Staphylococcus aureus plasmid pI258 regulates transcription of this system in vitro. The CadC protein was overproduced in Escherichia coli cells and partially purified. Gel shift assays of the proposed cadA operator/promoter region DNA showed specific association with the CadC protein. Control arsenic resistance operator/promoter DNA from the same plasmid was not shifted by the CadC protein. Cd2+, Bi3+, and Pb2+ caused the release of CadC from DNA in gel retardation assays. DNase I footprinting measurements showed that the CadC protein specifically associated with and protected a region of operator/promoter DNA from nucleotide positions -7 to +14 relative to the start point of mRNA synthesis. Runoff transcription assays with the operator/promoter region of DNA (plus the first 69 nucleotides of the cadC gene) and purified E. coli RNA polymerase gave an mRNA product of the predicted size. Added CadC protein inhibited transcription in vitro.

This article has been cited by other articles:

• Chen, Y.-Y. M., Feng, C. W., Chiu, C. F., Burne, R. A. (2008). cadDX Operon of Streptococcus salivarius 57.I. Appl. Environ. Microbiol. 74: 1642-1645 [Abstract] [Full Text]  
• Banci, L., Bertini, I., Cantini, F., Ciofi-Baffoni, S., Cavet, J. S., Dennison, C., Graham, A. I., Harvie, D. R., Robinson, N. J. (2007). NMR Structural Analysis of Cadmium Sensing by Winged Helix Repressor CmtR. J. Biol. Chem. 282: 30181-30188 [Abstract] [Full Text]  
• Ordonez, E., Letek, M., Valbuena, N., Gil, J. A., Mateos, L. M. (2005). Analysis of Genes Involved in Arsenic Resistance in Corynebacterium glutamicum ATCC 13032. Appl. Environ. Microbiol. 71: 6206-6215 [Abstract] [Full Text]  
• Naz, N., Young, H. K., Ahmed, N., Gadd, G. M. (2005). Cadmium Accumulation and DNA Homology with Metal Resistance Genes in Sulfate-Reducing Bacteria. Appl. Environ. Microbiol. 71: 4610-4618 [Abstract] [Full Text]  
• Rensing, C. (2005). Form and Function in Metal-Dependent Transcriptional Regulation: Dawn of the Enlightenment. J. Bacteriol. 187: 3909-3912 [Full Text]  
• Ye, J., Kandegedara, A., Martin, P., Rosen, B. P. (2005). Crystal Structure of the Staphylococcus aureus pI258 CadC Cd(II)/Pb(II)/Zn(II)-Responsive Repressor. J. Bacteriol. 187: 4214-4221 [Abstract] [Full Text]  
• Dodge, A. G., Wackett, L. P. (2005). Metabolism of Bismuth Subsalicylate and Intracellular Accumulation of Bismuth by Fusarium sp. Strain BI. Appl. Environ. Microbiol. 71: 876-882 [Abstract] [Full Text]  
• Liu, T., Nakashima, S., Hirose, K., Shibasaka, M., Katsuhara, M., Ezaki, B., Giedroc, D. P., Kasamo, K. (2004). A Novel Cyanobacterial SmtB/ArsR Family Repressor Regulates the Expression of a CPx-ATPase and a Metallothionein in Response to Both Cu(I)/Ag(I) and Zn(II)/Cd(II). J. Biol. Chem. 279: 17810-17818 [Abstract] [Full Text]  
• Cavet, J. S., Graham, A. I., Meng, W., Robinson, N. J. (2003). A Cadmium-Lead-sensing ArsR-SmtB Repressor with Novel Sensory Sites: COMPLEMENTARY METAL DISCRIMINATION BY NMTR AND CMTR IN A COMMON CYTOSOL. J. Biol. Chem. 278: 44560-44566 [Abstract] [Full Text]  
• Pennella, M. A., Shokes, J. E., Cosper, N. J., Scott, R. A., Giedroc, D. P. (2003). Bioinorganic Chemistry Special Feature: Structural elements of metal selectivity in metal sensor proteins. Proc. Natl. Acad. Sci. USA 100: 3713-3718 [Abstract] [Full Text]  
• Schirawski, J., Hagens, W., Fitzgerald, G. F., van Sinderen, D. (2002). Molecular Characterization of Cadmium Resistance in Streptococcus thermophilus Strain 4134: an Example of Lateral Gene Transfer. Appl. Environ. Microbiol. 68: 5508-5516 [Abstract] [Full Text]  
• Wong, M. D., Lin, Y.-F., Rosen, B. P. (2002). The Soft Metal Ion Binding Sites in the Staphylococcus aureus pI258 CadC Cd(II)/Pb(II)/Zn(II)-responsive Repressor Are Formed between Subunits of the Homodimer. J. Biol. Chem. 277: 40930-40936 [Abstract] [Full Text]  
• Cavet, J. S., Meng, W., Pennella, M. A., Appelhoff, R. J., Giedroc, D. P., Robinson, N. J. (2002). A Nickel-Cobalt-sensing ArsR-SmtB Family Repressor. CONTRIBUTIONS OF CYTOSOL AND EFFECTOR BINDING SITES TO METAL SELECTIVITY. J. Biol. Chem. 277: 38441-38448 [Abstract] [Full Text]  
• Roane, T. M., Josephson, K. L., Pepper, I. L. (2001). Dual-Bioaugmentation Strategy To Enhance Remediation of Cocontaminated Soil. Appl. Environ. Microbiol. 67: 3208-3215 [Abstract] [Full Text]  
• Lee, S.-W., Glickmann, E., Cooksey, D. A. (2001). Chromosomal Locus for Cadmium Resistance in Pseudomonas putida Consisting of a Cadmium-Transporting ATPase and a MerR Family Response Regulator. Appl. Environ. Microbiol. 67: 1437-1444 [Abstract] [Full Text]  
• Vats, N., Lee, S. F. (2001). Characterization of a copper-transport operon, copYAZ, from Streptococcus mutans. Microbiology 147: 653-662 [Abstract] [Full Text]  
• O' Sullivan, D., Ross, R. P., Twomey, D. P., Fitzgerald, G. F., Hill, C., Coffey, A. (2001). Naturally Occurring Lactococcal Plasmid pAH90 Links Bacteriophage Resistance and Mobility Functions to a Food-Grade Selectable Marker. Appl. Environ. Microbiol. 67: 929-937 [Abstract] [Full Text]  
• Turner, M. S., Helmann, J. D. (2000). Mutations in Multidrug Efflux Homologs, Sugar Isomerases, and Antimicrobial Biosynthesis Genes Differentially Elevate Activity of the sigma X and sigma W Factors in Bacillus subtilis. J. Bacteriol. 182: 5202-5210 [Abstract] [Full Text]  
• Xiong, A., Singh, V. K., Cabrera, G., Jayaswal, R. K. (2000). Molecular characterization of the ferric-uptake regulator, Fur, from Staphylococcus aureus. Microbiology 146: 659-668 [Abstract] [Full Text]  
• Rutherford, J. C., Cavet, J. S., Robinson, N. J. (1999). Cobalt-dependent Transcriptional Switching by a Dual-effector MerR-like Protein Regulates a Cobalt-exporting Variant CPx-type ATPase. J. Biol. Chem. 274: 25827-25832 [Abstract] [Full Text]  
• Crupper, S. S., Worrell, V., Stewart, G. C., Iandolo, J. J. (1999). Cloning and Expression of cadD, a New Cadmium Resistance Gene of Staphylococcus aureus. J. Bacteriol. 181: 4071-4075 [Abstract] [Full Text]  
• Rensing, C., Sun, Y., Mitra, B., Rosen, B. P. (1998). Pb(II)-translocating P-type ATPases. J. Biol. Chem. 273: 32614-32617 [Abstract] [Full Text]  
• Thelwell, C., Robinson, N. J., Turner-Cavet, J. S. (1998). An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter. Proc. Natl. Acad. Sci. USA 95: 10728-10733 [Abstract] [Full Text]  
• Williams, S. G., Carmel-Harel, O., Manning, P. A. (1998). A Functional Homolog of Escherichia coli NhaR in Vibrio cholerae. J. Bacteriol. 180: 762-765 [Abstract] [Full Text]  
• Rensing, C., Mitra, B., Rosen, B. P. (1997). The zntA gene of Escherichia coli encodes a Zn(II)-translocating P-type ATPase. Proc. Natl. Acad. Sci. USA 94: 14326-14331 [Abstract] [Full Text]  
• Strausak, D., Solioz, M. (1997). CopY Is a Copper-inducible Repressor of the Enterococcus hirae Copper ATPases. J. Biol. Chem. 272: 8932-8936 [Abstract] [Full Text]  
• Komeda, H., Kobayashi, M., Shimizu, S. (1996). A Novel Gene Cluster Including the Rhodococcus rhodochrous J1 nhlBA Genes Encoding a Low Molecular Mass Nitrile Hydratase (L-NHase) Induced by Its Reaction Product. J. Biol. Chem. 271: 15796-15802 [Abstract] [Full Text]