This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schweizer, H. P. Articles by Bacic, M. K. Search for Related Content PubMed PubMed Citation Articles by Schweizer, H. P. Articles by Bacic, M. K.

 Previous Article  |  Next Article 

J. Bacteriol., Aug 1995, 4801-4804, Vol 177, No. 16
Copyright © 1995, American Society for Microbiology

Identification of Pseudomonas aeruginosa glpM, whose gene product is required for efficient alginate biosynthesis from various carbon sources

HP Schweizer, C Po and MK Bacic
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Alberta, Canada.

In a mucB (algN) genetic background, insertion of an omega element approximately 200 bp downstream of glpD, encoding sn-glycerol-3- phosphate dehydrogenase from Pseudomonas aeruginosa, had an adverse effect on alginate biosynthesis from various carbon sources. The insertion inactivated glpM, a gene encoding a 12,040-M(r) hydrophobic protein containing 109 amino acids. This protein, which was expressed in a T7 RNA polymerase expression system, appears to be a cytoplasmic membrane protein.