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J. Bacteriol., 09 1995, 5364-5367, Vol 177, No. 18
Copyright © 1995, American Society for Microbiology

The MalK protein of the ATP-binding cassette transporter for maltose of Escherichia coli is accessible to protease digestion from the periplasmic side of the membrane

E Schneider, S Hunke and S Tebbe
Fachbereich Biologie/Chemie, Universitat Osnabruck, Germany.

The ATP-hydrolyzing subunit MalK of the ATP-binding cassette transporter for maltose of Escherichia coli is demonstrated to be accessible to digestion by proteinase K in right-side-out membrane vesicles. This finding suggests a partial transmembrane orientation of the protein.

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