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J. Bacteriol., 09 1995, 5379-5380, Vol 177, No. 18
Copyright © 1995, American Society for Microbiology

Cloning, sequencing, and expression of the araE gene of Klebsiella oxytoca 8017, which encodes arabinose-H+ symport activity

KP Shatwell, BM Charalambous, TP McDonald and PJ Henderson
Department of Biochemistry, University of Cambridge, United Kingdom.

Southern analysis of the genomic DNA from species of the family Enterobacteriaceae, using a probe derived from the Escherichia coli araE gene, which encodes an arabinose-H+ symporter, detected araE in Salmonella, Citrobacter, Klebsiella, and Enterobacter spp. The Klebsiella oxytoca araE gene was cloned, sequenced, and expressed to compare its properties with those of araE from E. coli.





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