This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Daly, M. J. Articles by Minton, K. W. Search for Related Content PubMed PubMed Citation Articles by Daly, M. J. Articles by Minton, K. W.

 Previous Article  |  Next Article 

J. Bacteriol., Oct 1995, 5495-5505, Vol 177, No. 19
Copyright © 1995, American Society for Microbiology

Interchromosomal recombination in the extremely radioresistant bacterium Deinococcus radiodurans

MJ Daly and KW Minton
Department of Pathology, F. E. Hebert School of Medicine, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814- 4799, USA.

Deinococcus radiodurans and other members of the genus Deinococcus are remarkable for their extreme resistance to ionizing radiation and many other agents that damage DNA. We have recently shown that recombinational processes participate in interplasmidic repair following in vivo irradiation. We now present direct studies on interchromosomal recombination among chromosomes irradiated in vivo during stationary phase (four chromosomes per cell). Following an exposure to 1.75 Mrad (the dose required to achieve a survival of 37%, which degrades the cells' four chromosomes into about 500 fragments), we determined that there may be as many as 175 crossovers per chromosome (700 crossovers per nucleoid) undergoing repair. In addition, these studies suggest that many of the crossovers occurring during repair are nonreciprocal.

This article has been cited by other articles:

• Eltsov, M., Dubochet, J. (2006). Study of the Deinococcus radiodurans Nucleoid by Cryoelectron Microscopy of Vitreous Sections: Supplementary Comments.. J. Bacteriol. 188: 6053-6058 [Full Text]  
• Tzeng, L., Singer, M. (2005). DNA replication during sporulation in Myxococcus xanthus fruiting bodies. Proc. Natl. Acad. Sci. USA 102: 14428-14433 [Abstract] [Full Text]  
• Tanaka, M., Earl, A. M., Howell, H. A., Park, M.-J., Eisen, J. A., Peterson, S. N., Battista, J. R. (2004). Analysis of Deinococcus radiodurans's Transcriptional Response to Ionizing Radiation and Desiccation Reveals Novel Proteins That Contribute to Extreme Radioresistance. Genetics 168: 21-33 [Abstract] [Full Text]  
• Brim, H., Venkateswaran, A., Kostandarithes, H. M., Fredrickson, J. K., Daly, M. J. (2003). Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments. Appl. Environ. Microbiol. 69: 4575-4582 [Abstract] [Full Text]  
• Jolivet, E., Matsunaga, F., Ishino, Y., Forterre, P., Prieur, D., Myllykallio, H. (2003). Physiological Responses of the Hyperthermophilic Archaeon "Pyrococcus abyssi" to DNA Damage Caused by Ionizing Radiation. J. Bacteriol. 185: 3958-3961 [Abstract] [Full Text]  
• Liu, Y., Zhou, J., Omelchenko, M. V., Beliaev, A. S., Venkateswaran, A., Stair, J., Wu, L., Thompson, D. K., Xu, D., Rogozin, I. B., Gaidamakova, E. K., Zhai, M., Makarova, K. S., Koonin, E. V., Daly, M. J. (2003). Transcriptome dynamics of Deinococcus radiodurans recovering from ionizing radiation. Proc. Natl. Acad. Sci. USA 100: 4191-4196 [Abstract] [Full Text]  
• Levin-Zaidman, S., Englander, J., Shimoni, E., Sharma, A. K., Minton, K. W., Minsky, A. (2003). Ringlike Structure of the Deinococcus radiodurans Genome: A Key to Radioresistance?. Science 299: 254-256 [Abstract] [Full Text]  
• Mrazek, J. (2002). New technology may reveal mechanisms of radiation resistance in Deinococcus radiodurans. Proc. Natl. Acad. Sci. USA 99: 10943-10944 [Full Text]  
• Kim, J.-I., Sharma, A. K., Abbott, S. N., Wood, E. A., Dwyer, D. W., Jambura, A., Minton, K. W., Inman, R. B., Daly, M. J., Cox, M. M. (2002). RecA Protein from the Extremely Radioresistant Bacterium Deinococcus radiodurans: Expression, Purification, and Characterization. J. Bacteriol. 184: 1649-1660 [Abstract] [Full Text]  
• Karlin, S., Mrázek, J. (2001). Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage. Proc. Natl. Acad. Sci. USA 10.1073/pnas.081077598v1 [Abstract] [Full Text]  
• Makarova, K. S., Aravind, L., Wolf, Y. I., Tatusov, R. L., Minton, K. W., Koonin, E. V., Daly, M. J. (2001). Genome of the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans Viewed from the Perspective of Comparative Genomics. Microbiol. Mol. Biol. Rev. 65: 44-79 [Abstract] [Full Text]  
• Venkateswaran, A., McFarlan, S. C., Ghosal, D., Minton, K. W., Vasilenko, A., Makarova, K., Wackett, L. P., Daly, M. J. (2000). Physiologic Determinants of Radiation Resistance in Deinococcus radiodurans. Appl. Environ. Microbiol. 66: 2620-2626 [Abstract] [Full Text]  
• Fredrickson, J. K., Kostandarithes, H. M., Li, S. W., Plymale, A. E., Daly, M. J. (2000). Reduction of Fe(III), Cr(VI), U(VI), and Tc(VII) by Deinococcus radiodurans R1. Appl. Environ. Microbiol. 66: 2006-2011 [Abstract] [Full Text]  
• Zhang, Y.-M., Wong, T.-Y., Chen, L.-Y., Lin, C.-S., Liu, J.-K. (2000). Induction of a Futile Embden-Meyerhof-Parnas Pathway in Deinococcus radiodurans by Mn: Possible Role of the Pentose Phosphate Pathway in Cell Survival. Appl. Environ. Microbiol. 66: 105-112 [Abstract] [Full Text]  
• Karlin, S., Mrazek, J. (2001). Predicted highly expressed and putative alien genes of Deinococcus radiodurans and implications for resistance to ionizing radiation damage. Proc. Natl. Acad. Sci. USA 98: 5240-5245 [Abstract] [Full Text]