This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kruger, S. Articles by Hecker, M. Search for Related Content PubMed PubMed Citation Articles by Kruger, S. Articles by Hecker, M.

 Previous Article  |  Next Article 

J. Bacteriol., Oct 1995, 5590-5597, Vol 177, No. 19
Copyright © 1995, American Society for Microbiology

Regulation of the putative bglPH operon for aryl-beta-glucoside utilization in Bacillus subtilis

S Kruger and M Hecker
Institut fur Mikrobiologie und Molekularbiologie, Ernst-Moritz-Arndt- Universitat, Greifswald, Germany.

The expression of the putative operon bglPH of Bacillus subtilis was studied by using bglP'-lacZ transcriptional fusions. The bglP gene encodes an aryl-beta-glucoside-specific enzyme II of the phosphoenolpyruvate sugar:phosphotransferase system, whereas the bglH gene product functions as a phospho-beta-glucosidase. Expression of bglPH is regulated by at least two different mechanisms: (i) carbon catabolite repression and (ii) induction via an antitermination mechanism. Distinct deletions of the promoter region were created to determine cis-acting sites for regulation. An operatorlike structure partially overlapping the -35 box of the promoter of bglP appears to be the catabolite-responsive element of this operon. The motif is similar to that of amyO and shows no mismatches with respect to the consensus sequence established as the target of carbon catabolite repression in B. subtilis. Catabolite repression is abolished in both ccpA and ptsH1 mutants. The target of the induction by the substrate, salicin or arbutin, is a transcriptional terminator located downstream from the promoter of bglP. This structure is very similar to that of transcriptional terminators which regulate the induction of the B. subtilis sacB gene, the sacPA operon, and the Escherichia coli bgl operon. The licT gene product, a member of the BglG-SacY family of antitermination proteins, is essential for the induction process. Expression of bglP is under the negative control of its own gene product. The general proteins of the phosphoenolpyruvate-dependent phosphotransferase system are required for bglP expression. Furthermore, the region upstream from bglP, which reveals a high AT content, exerts a negative regulatory effect on bglP expression.

This article has been cited by other articles:

• Perkins, A. E., Nicholson, W. L. (2008). Uncovering New Metabolic Capabilities of Bacillus subtilis Using Phenotype Profiling of Rifampin-Resistant rpoB Mutants. J. Bacteriol. 190: 807-814 [Abstract] [Full Text]  
• Schilling, O., Herzberg, C., Hertrich, T., Vorsmann, H., Jessen, D., Hubner, S., Titgemeyer, F., Stulke, J. (2006). Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples. Nucleic Acids Res 34: 6102-6115 [Abstract] [Full Text]  
• Deutscher, J., Francke, C., Postma, P. W. (2006). How Phosphotransferase System-Related Protein Phosphorylation Regulates Carbohydrate Metabolism in Bacteria. Microbiol. Mol. Biol. Rev. 70: 939-1031 [Abstract] [Full Text]  
• Graille, M., Zhou, C.-Z., Receveur-Brechot, V., Collinet, B., Declerck, N., van Tilbeurgh, H. (2005). Activation of the LicT Transcriptional Antiterminator Involves a Domain Swing/Lock Mechanism Provoking Massive Structural Changes. J. Biol. Chem. 280: 14780-14789 [Abstract] [Full Text]  
• Schilling, O., Langbein, I., Muller, M., Schmalisch, M. H., Stulke, J. (2004). A protein-dependent riboswitch controlling ptsGHI operon expression in Bacillus subtilis: RNA structure rather than sequence provides interaction specificity. Nucleic Acids Res 32: 2853-2864 [Abstract] [Full Text]  
• Schmalisch, M. H., Bachem, S., Stulke, J. (2003). Control of the Bacillus subtilis Antiterminator Protein GlcT by Phosphorylation: ELUCIDATION OF THE PHOSPHORYLATION CHAIN LEADING TO INACTIVATION OF GlcT. J. Biol. Chem. 278: 51108-51115 [Abstract] [Full Text]  
• Kotrba, P., Inui, M., Yukawa, H. (2003). A single V317A or V317M substitution in Enzyme II of a newly identified {beta}-glucoside phosphotransferase and utilization system of Corynebacterium glutamicum R extends its specificity towards cellobiose. Microbiology 149: 1569-1580 [Abstract] [Full Text]  
• Lindner, C., Hecker, M., Le Coq, D., Deutscher, J. (2002). Bacillus subtilis Mutant LicT Antiterminators Exhibiting Enzyme I- and HPr-Independent Antitermination Affect Catabolite Repression of the bglPH Operon. J. Bacteriol. 184: 4819-4828 [Abstract] [Full Text]  
• Faure, D. (2002). The Family-3 Glycoside Hydrolases: from Housekeeping Functions to Host-Microbe Interactions. Appl. Environ. Microbiol. 68: 1485-1490 [Full Text]  
• Gosalbes, M. J., Esteban, C. D., Perez-Martinez, G. (2002). In vivo effect of mutations in the antiterminator LacT in Lactobacillus casei. Microbiology 148: 695-702 [Abstract] [Full Text]  
• Knezevic, I., Bachem, S., Sickmann, A., Meyer, H. E., Stulke, J., Hengstenberg, W. (2000). Regulation of the glucose-specific phosphotransferase system (PTS) of Staphylococcus carnosus by the antiterminator protein GlcT. Microbiology 146: 2333-2342 [Abstract] [Full Text]  
• Cote, C. K., Cvitkovitch, D., Bleiweis, A. S., Honeyman, A. L. (2000). A novel {beta}-glucoside-specific PTS locus from Streptococcus mutans that is not inhibited by glucose. Microbiology 146: 1555-1563 [Abstract] [Full Text]  
• Henstra, S. A., Duurkens, R. H., Robillard, G. T. (2000). Multiple Phosphorylation Events Regulate the Activity of the Mannitol Transcriptional Regulator MtlR of the Bacillus stearothermophilus Phosphoenolpyruvate-dependent Mannitol Phosphotransferase System. J. Biol. Chem. 275: 7037-7044 [Abstract] [Full Text]  
• Miwa, Y., Nakata, A., Ogiwara, A., Yamamoto, M., Fujita, Y. (2000). Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis. Nucleic Acids Res 28: 1206-1210 [Abstract] [Full Text]  
• Tobisch, S., Stülke, J., Hecker, M. (1999). Regulation of the lic Operon of Bacillus subtilis and Characterization of Potential Phosphorylation Sites of the LicR Regulator Protein by Site-Directed Mutagenesis. J. Bacteriol. 181: 4995-5003 [Abstract] [Full Text]  
• Gosalbes, M. J., Monedero, V., Pérez-Martínez, G. (1999). Elements Involved in Catabolite Repression and Substrate Induction of the Lactose Operon in Lactobacillus casei. J. Bacteriol. 181: 3928-3934 [Abstract] [Full Text]  
• Zalieckas, J. M., Wray, L. V. Jr., Fisher, S. H. (1999). trans-Acting Factors Affecting Carbon Catabolite Repression of the hut Operon in Bacillus subtilis. J. Bacteriol. 181: 2883-2888 [Abstract] [Full Text]  
• Boss, A., Nussbaum-Shochat, A., Amster-Choder, O. (1999). Characterization of the Dimerization Domain in BglG, an RNA-Binding Transcriptional Antiterminator from Escherichia coli. J. Bacteriol. 181: 1755-1766 [Abstract] [Full Text]  
• Zalieckas, J. M., Wray, L. V. Jr., Fisher, S. H. (1998). Expression of the Bacillus subtilis acsA Gene: Position and Sequence Context Affect cre-Mediated Carbon Catabolite Repression. J. Bacteriol. 180: 6649-6654 [Abstract] [Full Text]  
• Turinsky, A. J., Grundy, F. J., Kim, J.-H., Chambliss, G. H., Henkin, T. M. (1998). Transcriptional Activation of the Bacillus subtilis ackA Gene Requires Sequences Upstream of the Promoter. J. Bacteriol. 180: 5961-5967 [Abstract] [Full Text]  
• Marasco, R., Muscariello, L., Varcamonti, M., De Felice, M., Sacco, M. (1998). Expression of the bglH Gene of Lactobacillus plantarum Is Controlled by Carbon Catabolite Repression. J. Bacteriol. 180: 3400-3404 [Abstract] [Full Text]  
• Krispin, O., Allmansberger, R. (1998). . J. Bacteriol. 180: 3250-3252 [Abstract]  
• Idelson, M., Amster-Choder, O. (1998). SacY, a Transcriptional Antiterminator from Bacillus subtilis, Is Regulated by Phosphorylation In Vivo. J. Bacteriol. 180: 660-666 [Abstract] [Full Text]  
• Chen, Q., Engelberg-Kulka, H., Amster-Choder, O. (1997). The Localization of the Phosphorylation Site of BglG, the Response Regulator of the Escherichia coli bgl Sensory System. J. Biol. Chem. 272: 17263-17268 [Abstract] [Full Text]  
• Tortosa, P., Aymerich, S., Lindner, C., Saier Jr., M. H., Reizer, J., Le Coq, D. (1997). Multiple Phosphorylation of SacY, a Bacillus subtilis Transcriptional Antiterminator Negatively Controlled by the Phosphotransferase System. J. Biol. Chem. 272: 17230-17237 [Abstract] [Full Text]  
• Arnaud, M., Debarbouille, M., Rapoport, G., Saier, M. H. Jr., Reizer, J. (1996). In Vitro Reconstitution of Transcriptional Antitermination by the SacT and SacY Proteins of Bacillus subtilis. J. Biol. Chem. 271: 18966-18972 [Abstract] [Full Text]