This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilkinson, S. R. Articles by Young, M. Search for Related Content PubMed PubMed Citation Articles by Wilkinson, S. R. Articles by Young, M.

 Previous Article  |  Next Article 

J. Bacteriol., Jan 1995, 439-448, Vol 177, No. 2
Copyright © 1995, American Society for Microbiology

Physical map of the Clostridium beijerinckii (formerly Clostridium acetobutylicum) NCIMB 8052 chromosome

SR Wilkinson and M Young
Institute of Biological Sciences, University of Wales, Aberystwyth, Dyfed, United Kingdom.

A combined physical and genetic map of the single, circular, 6.7-Mbp chromosome of the NCIMB 8052 strain of Clostridium beijerinckii (formerly Clostridium acetobutylicum) has been constructed by using a combination of cloned DNA fragments as hybridization probes and a bank of strains harboring insertions of the conjugative transposon Tn1545. The positions of 81 restriction endonuclease cleavage sites and 32 genes have been determined. Eight genes concerned with solventogenic fermentation are found at three different locations. The chromosome contains at least 13 rrn operons, 11 of which have been located on the map. Their transcriptional orientation diverges from the presumed location of the replication origin.

This article has been cited by other articles:

• Keis, S., Sullivan, J. T., Jones, D. T. (2001). Physical and genetic map of the Clostridium saccharobutylicum (formerly Clostridium acetobutylicum) NCP 262 chromosome. Microbiology 147: 1909-1922 [Abstract] [Full Text]  
• Chen, C.-K., Blaschek, H. P. (1999). Effect of Acetate on Molecular and Physiological Aspects of Clostridium beijerinckii NCIMB 8052 Solvent Production and Strain Degeneration. Appl. Environ. Microbiol. 65: 499-505 [Abstract] [Full Text]  
• Evans, V. J., Liyanage, H., Ravagnani, A., Young, M., Kashket, E. R. (1998). Truncation of Peptide Deformylase Reduces the Growth Rate and Stabilizes Solvent Production in Clostridium beijerinckii NCIMB 8052. Appl. Environ. Microbiol. 64: 1780-1785 [Abstract] [Full Text]