This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Meurer, G. Articles by Hutchinson, C. R. Search for Related Content PubMed PubMed Citation Articles by Meurer, G. Articles by Hutchinson, C. R.

 Previous Article  |  Next Article 

J. Bacteriol., 01 1995, 477-481, Vol 177, No. 2
Copyright © 1995, American Society for Microbiology

Functional analysis of putative beta-ketoacyl:acyl carrier protein synthase and acyltransferase active site motifs in a type II polyketide synthase of Streptomyces glaucescens

G Meurer and CR Hutchinson
School of Pharmacy, University of Wisconsin, Madison 53706.

The significance of potential active site motifs for acyltransferase and beta-ketoacyl:acyl carrier protein synthase regions within the TcmK protein was investigated by determining the effects of mutations in the proposed active sites on the production of tetracenomycins F2 and C. In a Streptomyces glaucescens tcmGHI JKLMNO null mutant, plasmids carrying the S351A mutation produced high amounts of tetracenomycin F2 but plasmids carrying the C173A or C173S mutation or the H350L-S351A double mutation produced no detectable amount of any known intermediate. In a tcmK mutant, plasmids with the S351A mutation restored high production of tetracenomycin C and plasmids carrying the other mutations were able to complement the chromosomal defect to some extent. None of the mutations affected the amount of TcmK produced.

This article has been cited by other articles:

• Aparicio, J. F., Colina, A. J., Ceballos, E., Martin, J. F. (1999). The Biosynthetic Gene Cluster for the 26-Membered Ring Polyene Macrolide Pimaricin. A NEW POLYKETIDE SYNTHASE ORGANIZATION ENCODED BY TWO SUBCLUSTERS SEPARATED BY FUNCTIONALIZATION GENES. J. Biol. Chem. 274: 10133-10139 [Abstract] [Full Text]  
• Fuchs, R. P. P., Napolitano, R. L. (1998). Inactivation of DNA proofreading obviates the need for SOS induction in frameshift mutagenesis. Proc. Natl. Acad. Sci. USA 95: 13114-13119 [Abstract] [Full Text]