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J. Bacteriol., 10 1995, 6015-6017, Vol 177, No. 20
Copyright © 1995, American Society for Microbiology

Distance between alleles as a determinant of linkage in natural transformation of Acinetobacter calcoaceticus

DU Kloos, AA DiMarco, DA Elsemore, KN Timmis and LN Ornston
Biology Department, Yale University, New Haven, Connecticut 06520, USA.

Cotransformation frequencies of 16, 39, 51, and 60% were observed when donor alleles were separated by distances of 9.2, 7.4, 6.3, and 5.1 kb, respectively, in donor Acinetobacter calcoaceticus DNA. A different and unexpected pattern was observed when the distance between recipient alleles was reduced from 9.2 to 5.1 kb. Ligation of unlinked chromosomal DNA fragments allowed them to be linked genetically through natural transformation.

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