This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ehrhardt, D. W. Articles by Long, S. R. Search for Related Content PubMed PubMed Citation Articles by Ehrhardt, D. W. Articles by Long, S. R.

 Previous Article  |  Next Article 

J. Bacteriol., Nov 1995, 6237-6245, Vol 177, No. 21
Copyright © 1995, American Society for Microbiology

In vitro sulfotransferase activity of NodH, a nodulation protein of Rhizobium meliloti required for host-specific nodulation

DW Ehrhardt, EM Atkinson, KF Faull, DI Freedberg, DP Sutherlin, R Armstrong and SR Long
Gilbert Laboratory, Howard Hughes Medical Institute, Stanford University, California 94305-5020, USA.

Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is modified by host- specific gene products. Here we show direct in vitro evidence that Rhizobium meliloti NodH, a host-specific nodulation gene, catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to the terminal 6-O position of Nod factors, and we show substrate requirements for the reaction. Our results indicate that polymerization of the chitooligosaccharide backbone likely precedes sulfation and that sulfation is not absolutely dependent on the presence or the particular structure of the N-acyl modification. NodH sulfation provides a tool for the enzymatic in vitro synthesis of novel Nod factors, or putative Nod factors intermediates, with high specific activity.

This article has been cited by other articles:

• Keating, D. H. (2007). Sinorhizobium meliloti SyrA Mediates the Transcriptional Regulation of Genes Involved in Lipopolysaccharide Sulfation and Exopolysaccharide Biosynthesis. J. Bacteriol. 189: 2510-2520 [Abstract] [Full Text]  
• Cronan, G. E., Keating, D. H. (2004). Sinorhizobium meliloti Sulfotransferase That Modifies Lipopolysaccharide. J. Bacteriol. 186: 4168-4176 [Abstract] [Full Text]  
• Pi, N., Yu, Y., Mougous, J. D., Leary, J. A. (2004). Observation of a hybrid random ping-pong mechanism of catalysis for NodST: A mass spectrometry approach. Protein Sci. 13: 903-912 [Abstract] [Full Text]  
• Gonzalez, J. E., Marketon, M. M. (2003). Quorum Sensing in Nitrogen-Fixing Rhizobia. Microbiol. Mol. Biol. Rev. 67: 574-592 [Abstract] [Full Text]  
• Snoeck, C., Verreth, C., Hernandez-Lucas, I., Martinez-Romero, E., Vanderleyden, J. (2003). Identification of a Third Sulfate Activation System in Sinorhizobium sp. Strain BR816: the CysDN Sulfate Activation Complex. Appl. Environ. Microbiol. 69: 2006-2014 [Abstract] [Full Text]  
• Mougous, J. D., Leavell, M. D., Senaratne, R. H., Leigh, C. D., Williams, S. J., Riley, L. W., Leary, J. A., Bertozzi, C. R. (2002). Discovery of sulfated metabolites in mycobacteria with a genetic and mass spectrometric approach. Proc. Natl. Acad. Sci. USA 99: 17037-17042 [Abstract] [Full Text]  
• Keating, D. H., Willits, M. G., Long, S. R. (2002). A Sinorhizobium meliloti Lipopolysaccharide Mutant Altered in Cell Surface Sulfation. J. Bacteriol. 184: 6681-6689 [Abstract] [Full Text]  
• Southwick, A. M., Wang, L.-X., Long, S. R., Lee, Y. C. (2002). Activity of Sinorhizobium meliloti NodAB and NodH Enzymes on Thiochitooligosaccharides. J. Bacteriol. 184: 4039-4043 [Abstract] [Full Text]  
• Rivera-Marrero, C. A., Ritzenthaler, J. D., Newburn, S. A., Roman, J., Cummings, R. D. (2002). Molecular cloning and expression of a novel glycolipid sulfotransferase in Mycobacterium tuberculosis. Microbiology 148: 783-792 [Abstract] [Full Text]  
• Barnett, M. J., Fisher, R. F., Jones, T., Komp, C., Abola, A. P., Barloy-Hubler, F., Bowser, L., Capela, D., Galibert, F., Gouzy, J., Gurjal, M., Hong, A., Huizar, L., Hyman, R. W., Kahn, D., Kahn, M. L., Kalman, S., Keating, D. H., Palm, C., Peck, M. C., Surzycki, R., Wells, D. H., Yeh, K.-C., Davis, R. W., Federspiel, N. A., Long, S. R. (2001). Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid. Proc. Natl. Acad. Sci. USA 10.1073/pnas.161294798v1 [Abstract] [Full Text]  
• D'Haeze, W., Mergaert, P., Prome, J.-C., Holsters, M. (2000). Nod Factor Requirements for Efficient Stem and Root Nodulation of the Tropical Legume Sesbania rostrata. J. Biol. Chem. 275: 15676-15684 [Abstract] [Full Text]  
• Perret, X., Staehelin, C., Broughton, W. J. (2000). Molecular Basis of Symbiotic Promiscuity. Microbiol. Mol. Biol. Rev. 64: 180-201 [Abstract] [Full Text]  
• Abola, A. P., Willits, M. G., Wang, R. C., Long, S. R. (1999). Reduction of Adenosine-5'-Phosphosulfate Instead of 3'-Phosphoadenosine-5'-Phosphosulfate in Cysteine Biosynthesis by Rhizobium meliloti and Other Members of the Family Rhizobiaceae. J. Bacteriol. 181: 5280-5287 [Abstract] [Full Text]  
• Itin, C., Rancano, C., Nakajima, Y., Pfeffer, S. R. (1997). A Novel Assay Reveals a Role for Soluble N-Ethylmaleimide-sensitive Fusion Attachment Protein in Mannose 6-Phosphate Receptor Transport from Endosomes to the Trans Golgi Network. J. Biol. Chem. 272: 27737-27744 [Abstract] [Full Text]  
• Roche, P., Maillet, F., Plazanet, C., Debelle, F., Ferro, M., Truchet, G., Prome, J.-C., Denarie, J. (1996). The common nodABC genes of Rhizobium meliloti are host-range determinants. Proc. Natl. Acad. Sci. USA 93: 15305-15310 [Abstract] [Full Text]  
• Barnett, M. J., Fisher, R. F., Jones, T., Komp, C., Abola, A. P., Barloy-Hubler, F., Bowser, L., Capela, D., Galibert, F., Gouzy, J., Gurjal, M., Hong, A., Huizar, L., Hyman, R. W., Kahn, D., Kahn, M. L., Kalman, S., Keating, D. H., Palm, C., Peck, M. C., Surzycki, R., Wells, D. H., Yeh, K.-C., Davis, R. W., Federspiel, N. A., Long, S. R. (2001). From the Cover: Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid. Proc. Natl. Acad. Sci. USA 98: 9883-9888 [Abstract] [Full Text]