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J. Bacteriol., 11 1995, 6282-6285, Vol 177, No. 21
Copyright © 1995, American Society for Microbiology

Mass spectrometric analysis of chitin oligosaccharides produced by Rhizobium NodC protein in Escherichia coli

E Kamst, KM van der Drift, JE Thomas-Oates, BJ Lugtenberg and HP Spaink
Clusius Laboratory, Institute of Molecular Plant Sciences, Leiden University, The Netherlands.

A system for studying the in vivo activity of Rhizobium NodC protein in Escherichia coli has been developed. Using thin-layer chromatography, high-performance liquid chromatography, and mass spectrometry, we show that in this system R. leguminosarum bv. viciae NodC protein directs the synthesis of chitinpentaose, chitintetraose, chitintriose, and two as yet unidentified modified chitin oligosaccharides.

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