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J. Bacteriol., Nov 1995, 6301-6303, Vol 177, No. 21
Copyright © 1995, American Society for Microbiology

Instability of Rickettsia prowazekii RNA polymerase-promoter complexes

LP Aniskovitch and HH Winkler
Department of Microbiology and Immunology, University of South Alabama College of Medicine, Mobile 36688, USA.

The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. The R. prowazekii core played the major role in determining heparin sensitivity.

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