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J. Bacteriol., Nov 1995, 6309-6312, Vol 177, No. 21
Copyright © 1995, American Society for Microbiology

Antigenic determinants of the membrane-bound hydrogenase in Alcaligenes eutrophus are exposed toward the periplasm

K Eismann, K Mlejnek, D Zipprich, M Hoppert, H Gerberding and F Mayer
Institut fur Mikrobiologie, Georg-August-Universitat zu Gottingen, Germany.

Electron microscopic immunogold labeling experiments were performed with ultrathin sections of plasmolyzed cells of Alcaligenes eutrophus and "whole-mount" samples of spheroplasts and protoplasts. They demonstrated that antigenic determinants of the membrane-bound hydrogenase are exposed, at the outside of the cytoplasmic membrane, to the periplasm.


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