This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Norregaard-Madsen, M. Articles by Valentin-Hansen, P. Search for Related Content PubMed PubMed Citation Articles by Norregaard-Madsen, M. Articles by Valentin-Hansen, P.

 Previous Article  |  Next Article 

J. Bacteriol., 11 1995, 6456-6461, Vol 177, No. 22
Copyright © 1995, American Society for Microbiology

Organization and transcriptional regulation of the Escherichia coli K- 12 D-serine tolerance locus

M Norregaard-Madsen, E McFall and P Valentin-Hansen
Department of Molecular Biology, Odense University, Denmark.

We have reinvestigated the genetic organization and the transcription regulation of the dsd operon of Escherichia coli. By combining genetic and biochemical studies, it is demonstrated that the regulatory region of the operon and the gene encoding the specific regulator of D-serine tolerance (dsdC) had been misplaced in previous work on the dsd system. Also, the previous erroneous DNA sequence of the dsdC gene has been corrected. It turned out that an additional gene (dsdX) is present immediately upstream of dsdA (encoding D-serine deaminase) and that dsdC is located adjacent to dsdX. The dsdXA genes are cotranscribed from a common promoter region present in the dsdX-dsdC intercistronic region. The DsdC activator belongs to the LysR-type of transcriptional regulators and is absolutely required for dsdA expression. Additionally, the activity of the dsdXA promoter depends on the cyclic AMP receptor protein, and the two activators act in concert to synergistically activate transcription.

This article has been cited by other articles:

• Anfora, A. T., Haugen, B. J., Roesch, P., Redford, P., Welch, R. A. (2007). Roles of Serine Accumulation and Catabolism in the Colonization of the Murine Urinary Tract by Escherichia coli CFT073. Infect. Immun. 75: 5298-5304 [Abstract] [Full Text]  
• Haugen, B. J., Pellett, S., Redford, P., Hamilton, H. L., Roesch, P. L., Welch, R. A. (2007). In Vivo Gene Expression Analysis Identifies Genes Required for Enhanced Colonization of the Mouse Urinary Tract by Uropathogenic Escherichia coli Strain CFT073 dsdA. Infect. Immun. 75: 278-289 [Abstract] [Full Text]  
• Moritz, R. L., Welch, R. A. (2006). The Escherichia coli argW-dsdCXA Genetic Island Is Highly Variable, and E. coli K1 Strains Commonly Possess Two Copies of dsdCXA.. J. Clin. Microbiol. 44: 4038-4048 [Abstract] [Full Text]  
• Anfora, A. T., Welch, R. A. (2006). DsdX Is the Second D-Serine Transporter in Uropathogenic Escherichia coli Clinical Isolate CFT073.. J. Bacteriol. 188: 6622-6628 [Abstract] [Full Text]  
• Xie, Y., Yao, Y., Kolisnychenko, V., Teng, C.-H., Kim, K. S. (2006). HbiF Regulates Type 1 Fimbriation Independently of FimB and FimE. Infect. Immun. 74: 4039-4047 [Abstract] [Full Text]  
• Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., Lengeler, J. W. (2002). Adaptation of Sucrose Metabolism in the Escherichia coli Wild-Type Strain EC3132{dagger}. J. Bacteriol. 184: 5307-5316 [Abstract] [Full Text]  
• Berlyn, M. K. B. (1998). Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map. Microbiol. Mol. Biol. Rev. 62: 814-984 [Abstract] [Full Text]