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J. Bacteriol., 11 1995, 6666-6669, Vol 177, No. 22
Copyright © 1995, American Society for Microbiology

Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene

JW Vrijbloed, J van Hylckama Vlieg, NM van der Put, GI Hessels and L Dijkhuizen
Department of Microbiology, University of Groningen, Haren, The Netherlands.

An efficient restriction barrier for methylated DNA in the actinomycete Amycolatopsis methanolica could be avoided by using a nonmethylating Escherichia coli strain for DNA isolations. The A. methanolica prephenate dehydratase gene was cloned from a gene bank in a pMEA300- derived shuttle vector in E. coli and characterized.

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