This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by de la Cruz, J. Articles by Romero, L. C. Search for Related Content PubMed PubMed Citation Articles by de la Cruz, J. Articles by Romero, L. C.

 Previous Article  |  Next Article 

J. Bacteriol., Dec 1995, 6937-6945, Vol 177, No. 23
Copyright © 1995, American Society for Microbiology

A novel endo-beta-1,3-glucanase, BGN13.1, involved in the mycoparasitism of Trichoderma harzianum

J de la Cruz, JA Pintor-Toro, T Benitez, A Llobell and LC Romero
Instituto de Bioquimica Vegetal y Fotosintesis, Consejo Superior de Investigaciones Cientificas y Universidad de Sevilla, Spain.

The mycoparasitic fungus Trichoderma harzianum CECT 2413 produces at least three extracellular beta-1,3-glucanases. The most basic of these extracellular enzymes, named BGN13.1, was expressed when either fungal cell wall polymers or autoclaved mycelia from different fungi were used as the carbon source. BGN13.1 was purified to electrophoretic homogeneity and was biochemically characterized. The enzyme was specific for beta-1,3 linkages and has an endolytic mode of action. A synthetic oligonucleotide primer based on the sequence of an internal peptide was designed to clone the cDNA corresponding to BGN13.1. The deduced amino acid sequence predicted a molecular mass of 78 kDa for the mature protein. Analysis of the amino acid sequence indicates that the enzyme contains three regions, one N-terminal leader sequence; another, nondefined sequence; and one cysteine-rich C-terminal sequence. Sequence comparison shows that this beta-1,3-glucanase, first described for filamentous fungi, belongs to a family different from that of its previously described bacterial, yeast, and plant counterparts. Enzymatic-activity, protein, and mRNA data indicated that bgn13.1 is repressed by glucose and induced by either fungal cell wall polymers or autoclaved yeast cells and mycelia. Finally, experimental evidence showed that the enzyme hydrolyzes yeast and fungal cell walls.

This article has been cited by other articles:

• Williams, J., Clarkson, J. M., Mills, P. R., Cooper, R. M. (2003). Saprotrophic and Mycoparasitic Components of Aggressiveness of Trichoderma harzianum Groups toward the Commercial Mushroom Agaricus bisporus. Appl. Environ. Microbiol. 69: 4192-4199 [Abstract] [Full Text]  
• Thornton, C. R., Pitt, D., Wakley, G. E., Talbot, N. J. (2002). Production of a monoclonal antibody specific to the genus Trichoderma and closely related fungi, and its use to detect Trichoderma spp. in naturally infested composts. Microbiology 148: 1263-1279 [Abstract] [Full Text]  
• Hong, T.-Y., Cheng, C.-W., Huang, J.-W., Meng, M. (2002). Isolation and biochemical characterization of an endo-1,3-{beta}-glucanase from Streptomyces sioyaensis containing a C-terminal family 6 carbohydrate-binding module that binds to 1,3-{beta}-glucan. Microbiology 148: 1151-1159 [Abstract] [Full Text]  
• Donzelli, B. G. G., Harman, G. E. (2001). Interaction of Ammonium, Glucose, and Chitin Regulates the Expression of Cell Wall-Degrading Enzymes in Trichoderma atroviride Strain P1. Appl. Environ. Microbiol. 67: 5643-5647 [Abstract] [Full Text]  
• Ait-Lahsen, H., Soler, A., Rey, M., de la Cruz, J., Monte, E., Llobell, A. (2001). An Antifungal Exo-alpha -1,3-Glucanase (AGN13.1) from the Biocontrol Fungus Trichoderma harzianum. Appl. Environ. Microbiol. 67: 5833-5839 [Abstract] [Full Text]  
• Giczey, G., Kerényi, Z., Fülöp, L., Hornok, L. (2001). Expression of cmg1, an Exo-{beta}-1,3-Glucanase Gene from Coniothyrium minitans, Increases during Sclerotial Parasitism. Appl. Environ. Microbiol. 67: 865-871 [Abstract] [Full Text]  
• Nogawa, M., Yatsui, K., Tomioka, A., Okada, H., Morikawa, Y. (1999). An alpha -L-Arabinofuranosidase from Trichoderma reesei Containing a Noncatalytic Xylan-Binding Domain. Appl. Environ. Microbiol. 65: 3964-3968 [Abstract] [Full Text]  
• Vázquez-Garcidueñas, S., Leal-Morales, C. A., Herrera-Estrella, A. (1998). Analysis of the beta -1,3-Glucanolytic System of the Biocontrol Agent Trichoderma harzianum. Appl. Environ. Microbiol. 64: 1442-1446 [Abstract] [Full Text]  
• Gueguen, Y., Voorhorst, W. G. B., van der Oost, J., de Vos, W. M. (1997). Molecular and Biochemical Characterization of an Endo-beta -1,3-glucanase of the Hyperthermophilic Archaeon Pyrococcus furiosus. J. Biol. Chem. 272: 31258-31264 [Abstract] [Full Text]