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J. Bacteriol., Dec 1995, 7271-7274, Vol 177, No. 24
Copyright © 1995, American Society for Microbiology

Physical map of the Bartonella bacilliformis genome

CM Krueger, KL Marks and GM Ihler
Department of Medical Biochemistry and Genetics, College of Medicine, Texas A&M University Health Science Center, College Station 77843-1114, USA.

The genome of Bartonella bacilliformis was shown to be a single circular DNA molecule of about 1,600 kbp having six NotI, four SfiI, and two CeuI sites. A physical map of the DNA was constructed by contour-clamped homogeneous electric field pulsed-field gel electrophoresis of DNA restriction fragments. rRNA operons, the invasion-associated locus, and a flagellin gene were located on the map by hybridization.


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