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J. Bacteriol., 12 1995, 7275-7279, Vol 177, No. 24
Copyright © 1995, American Society for Microbiology

Genetic basis of Neisseria gonorrhoeae lipooligosaccharide antigenic variation

RJ Danaher, JC Levin, D Arking, CL Burch, R Sandlin and DC Stein
Department of Microbiology, University of Maryland, College Park 20742, USA.

Neisseria gonorrhoeae lipooligosaccharide (LOS) undergoes antigenic variation at a high rate, and this variation can be monitored by changes in a strain's ability to bind LOS-specific monoclonal antibodies. We report here the cloning and identification of a gene, lsi-2, that can mediate this variation. The DNA sequence of lsi-2 has been determined for N. gonorrhoeae 1291, a strain that expresses a high- molecular-mass LOS, and a derivative of this strain, RS132L, that produces a truncated LOS. In the parental strain, lsi-2 contains a string of 12 guanines in the middle of its coding sequence. In cells that had antigenically varied to produce a truncated LOS, the number of guanines in lsi-2 was altered. Site-specific deletions were constructed to verify that expression of a 3.6-kDa LOS is due to alterations in lsi- 2.

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