J. Bacteriol., Dec 1995, 7280-7284, Vol 177, No. 24
Copyright © 1995, American Society for Microbiology

Tricistronic operon expression of the genes gcaD (tms), which encodes N- acetylglucosamine 1-phosphate uridyltransferase, prs, which encodes phosphoribosyl diphosphate synthetase, and ctc in vegetative cells of Bacillus subtilis

I Hilden, BN Krath and B Hove-Jensen
Department of Biological Chemistry, University of Copenhagen, Denmark.

The gcaD, prs, and ctc genes were shown to be organized as a tricistronic operon. The transcription of the prs gene, measured as phosphoribosyl diphosphate synthetase activity, and of the ctc gene, measured as beta-galactosidase activity specified by a ctc-lacZ protein fusion, were dependent on the promoter in front of the gcaD gene. Analysis of cDNA molecules prepared with gcaD-prs-ctc-specified mRNA as the template revealed an RNA transcript that encompassed all three cistrons.

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