J. Bacteriol., 02 1995, 1119-1120, Vol 177, No. 4
PD Sniegowski
Excision of phage Mu from Escherichia coli MCS2 was originally put forth as
a clear example of directed mutation. This claim was considerably weakened,
however, by subsequent evidence that Mu excision occurs during starvation
regardless of fitness consequences. Here, I use the classical
replica-plating technique to examine Mu excision during starvation of MCS2.
The results support the conclusion that Mu excision is not directed but is,
instead, nonspecifically induced by starvation.
Copyright © 1995, American Society for Microbiology
A test of the directed mutation hypothesis in Escherichia coli MCS2 using replica plating
Center for Microbial Ecology, Michigan State University, East Lansing 48824.
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