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J. Bacteriol., Feb 1995, 926-931, Vol 177, No. 4
Copyright © 1995, American Society for Microbiology

Activation of the dephosphorylation of nitrogen regulator I-phosphate of Escherichia coli

J Liu and B Magasanik
Department of Biology, Massachusetts Institute of Technology, Cambridge, 02139.

The transcription of sigma 54 RNA polymerase-dependent nitrogen- regulated genes is activated by nitrogen regulator I (NRI)-phosphate. The kinase NRII is responsible for the phosphorylation of NRI. It has been shown that NRII also has the ability to dephosphorylate NRI- phosphate but only when PII is present at a concentration greatly in excess of that of NRII. We have now shown that glutamate enables PII to stimulate the dephosphorylation of NRI-phosphate when present in equimolar concentration with NRII. This effect of glutamate appears to be a backup control that becomes effective when the normal regulation of PII activity is disabled.

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