This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lyons, E. M. Articles by Thiel, T. Search for Related Content PubMed PubMed Citation Articles by Lyons, E. M. Articles by Thiel, T.

 Previous Article  |  Next Article 

J. Bacteriol., 03 1995, 1570-1575, Vol 177, No. 6
Copyright © 1995, American Society for Microbiology

Characterization of nifB, nifS, and nifU genes in the cyanobacterium Anabaena variabilis: NifB is required for the vanadium-dependent nitrogenase

EM Lyons and T Thiel
Department of Biology, University of Missouri--St. Louis 63121.

Anabaena variabilis ATCC 29413 is a heterotrophic, nitrogen-fixing cyanobacterium containing both a Mo-dependent nitrogenase encoded by the nif genes and V-dependent nitrogenase encoded by the vnf genes. The nifB, nifS, and nifU genes of A. variabilis were cloned, mapped, and partially sequenced. The fdxN gene was between nifB and nifS. Growth and acetylene reduction assays using wild-type and mutant strains indicated that the nifB product (NifB) was required for nitrogen fixation not only by the enzyme encoded by the nif genes but also by the enzyme encoded by the vnf genes. Neither NifS nor NifU was essential for nitrogen fixation in A. variabilis.

This article has been cited by other articles:

• Toepel, J., Welsh, E., Summerfield, T. C., Pakrasi, H. B., Sherman, L. A. (2008). Differential Transcriptional Analysis of the Cyanobacterium Cyanothece sp. Strain ATCC 51142 during Light-Dark and Continuous-Light Growth. J. Bacteriol. 190: 3904-3913 [Abstract] [Full Text]  
• Weyman, P. D., Pratte, B., Thiel, T. (2008). Transcription of hupSL in Anabaena variabilis ATCC 29413 Is Regulated by NtcA and Not by Hydrogen. Appl. Environ. Microbiol. 74: 2103-2110 [Abstract] [Full Text]  
• Pratte, B. S., Eplin, K., Thiel, T. (2006). Cross-Functionality of Nitrogenase Components NifH1 and VnfH in Anabaena variabilis.. J. Bacteriol. 188: 5806-5811 [Abstract] [Full Text]  
• Pratte, B. S., Thiel, T. (2006). High-Affinity Vanadate Transport System in the Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 188: 464-468 [Abstract] [Full Text]  
• Choo, Q.-C., Samian, M.-R., Najimudin, N. (2003). Phylogeny and Characterization of Three nifH-Homologous Genes from Paenibacillus azotofixans. Appl. Environ. Microbiol. 69: 3658-3662 [Abstract] [Full Text]  
• Thiel, T., Pratte, B. (2001). Effect on Heterocyst Differentiation of Nitrogen Fixation in Vegetative Cells of the Cyanobacterium Anabaena variabilis ATCC 29413. J. Bacteriol. 183: 280-286 [Abstract] [Full Text]  
• Mihara, H., Kurihara, T., Yoshimura, T., Soda, K., Esaki, N. (1997). Cysteine Sulfinate Desulfinase, a NIFS-like Protein of Escherichia coli with Selenocysteine Lyase and Cysteine Desulfurase Activities. GENE CLONING, PURIFICATION, AND CHARACTERIZATION OF A NOVEL PYRIDOXAL ENZYME. J. Biol. Chem. 272: 22417-22424 [Abstract] [Full Text]