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J. Bacteriol., Mar 1995, 1610-1613, Vol 177, No. 6
Copyright © 1995, American Society for Microbiology

Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis

AJ van Asten, KA Zwaagstra, MF Baay, JG Kusters, JH Huis in't Veld and BA van der Zeijst
Department of Bacteriology, University of Utrecht, The Netherlands.

Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis.

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